I am preparing a protocol for SEM of a biofilm, but do not have easy access to 100% ethanol for the dehydration steps. Can 100% ethanol be substituted for 100% alcohol (95% EtOH, 5% methanol/isopropyl alcohol)? 95% ethanol generally is not anhydrous, and water has enough surface tension to potentially damage the specimen. I am also open to other suggestions if anyone has any. Thank you!!
95% EtOH and 5% methanol/isopropyl alcohol have worked for me for several samples for SEM, including bacteria.
Brian Lowrance
yes, we can substitute with 5% methanol/isopropyl alcohol. (time standardization is required).
subsequent treatment with HMDS is preferred for complete dehydration and maintenance of cell intactness.
Brent E Gowen if possible plz share your standardized protocol.
Thank you
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