Which primers sets(Sense two sets) Antisense(Two sets) I should use in conventional PCR? Two sense and two antisense primers what does it means?

Dear Researchers, I have read an article and there are two set of primer pairs. Sense primers and antisense primers. The paragraph from the article is as [ The partial DNA-dependent RNA...

31 December 2019 7,502 7 View

Can anyone help in SOD activity buffers?

In 3ml, how can I make all these buffers, I have read this paper for SOD activity, Please find below [The 3 ml reaction mixture contained 50 mM potassium phosphate buffer (pH 7.0), 0.5 mM...

06 July 2019 3,610 6 View

Is there any easy method to get Jasmonic acids from wheat plants?

Dear All, I need to check the growth promoting effects of some bacterial antagonists. I want to obtain the jasmonic acid and other growth promoting hormones. Then I want to do LCMS. Any step by...

05 June 2019 2,520 4 View

How to prepare solution for GC-MS/LC-MS/HPLC from the barley root exudates/leaf extracts?

I want to check some metabolites from the root exudates or leaf extracts. Than I want to check the metabolites concentration like Jasmonic acid, via GC-MS, LC-MS or HPLC. Can anyone explain me...

03 April 2019 3,325 5 View

Can anyone explain PERMANOVA pairwise results?

Dear All, Can anyone explain the pairwise permanova for the group 1 and Group 2. The data is given as below: Group 1 Group 2 Sample size Permutations pseudo-F p-value q-value A C 12 999...

11 December 2018 5,320 0 View

How can I count conidia on the PDA petriplates incubated for two weeks with haemocytomer?

I have a haemocytometer with 25 major grids and each grid is divided into 16 small grids, The dept is 0.1mm. I put 10 ml ddH20 in petriplates and rub to get spores. I take 10ul from that 10 ml...

01 February 2018 2,594 1 View

What is the definition of the strains in terms of fungi?

Dear All, I have read several papers like these: twelve strains of fungus Trichoderma harzianum were evaluated against Phytopathogen. So in these case what would be the definition of strains?

10 November 2017 6,147 10 View

Can someone recommend a proper procedure for extractions of antibiotics and peptides from the fungi?

Dear All, I want to extract antibiotics and peptides from a fungi. Is there any proper procedure to extract antibiotics from the fungi.

10 November 2017 6,618 3 View

Could not get a good and clear band in the Gell electrophoresis?

I run the Gell for DNA of some of samples. However some DNA bands were thinner some were more thicker in size and some of them were like smear from top to bottom. What could be the reason?

10 November 2017 9,762 4 View

How can I measure the percent inhibition of a plant pathogen caused by bi-control fungus?

Dear All, I have cultured two fungi one 9cm petri plates. The growth of biocontrol agent is very slow but it has potential to parasitize a plant pathogen. I want to measure the percent inhibition...

10 November 2017 4,934 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View