Dear All,
I have read several papers like these: twelve strains of fungus Trichoderma harzianum were evaluated against Phytopathogen. So in these case what would be the definition of strains?
Hello Aqleem,
In bacteriology, a strain refers to progenies of a pure, isolated culture and the succeeding descendants that can be cultured from it without contamination.
A strain, may also refer to a sub-variety of a microbe that is phenotypically and/or genotypically distinguishable from others. A fungus X may have multiple strains that are characteristically different in terms of certain genetic elements (e.g. rRNA gene). These differences are usually attributed to spontaneous and induced mutations, i.e., exposure of a fungus X to radiation, etc.
You may find this article helpful: http://www.microbiologyresearch.org/docserver/fulltext/jmm/49/5/mjm4905.397.pdf?expires=1511262341&id=id&accname=guest&checksum=811EA3BC5ACA7C4AE7CD8214EF82662F
Hope this helps.
Best,
Arizaldo
A strain, may be defined as here, is a genetic variant or sub-type of Trichoderma harzianum.
A strain is a genetic variant or sub-type of a micro-organism like fungus.
For example;
a "flu strain" is a certain biological form of the influenza or "flu" virus. Compare clade.
Hello Aqleem,
In bacteriology, a strain refers to progenies of a pure, isolated culture and the succeeding descendants that can be cultured from it without contamination.
A strain, may also refer to a sub-variety of a microbe that is phenotypically and/or genotypically distinguishable from others. A fungus X may have multiple strains that are characteristically different in terms of certain genetic elements (e.g. rRNA gene). These differences are usually attributed to spontaneous and induced mutations, i.e., exposure of a fungus X to radiation, etc.
You may find this article helpful: http://www.microbiologyresearch.org/docserver/fulltext/jmm/49/5/mjm4905.397.pdf?expires=1511262341&id=id&accname=guest&checksum=811EA3BC5ACA7C4AE7CD8214EF82662F
Hope this helps.
Best,
Arizaldo
Hello Aqleem,
A strain is an as homokaryotic mycelium than possible, obtained by direct single conidia/spore isolation or from subculture of a mycelium isolate. For sterile fungi, hyphal tip subcultures can minimize the risk of heterokaryosis.The latter can occur with conidia (eg Botrytis cinerea), mycelia (eg Thanetophorus cucumeris ) containing several, possibly genetically different, nuclei.
Thanks. I have another question related to aforementioned. Suppose some spore of same color are infecting other fungal structures in petri plates, Will these spores be called different strains.
They may be the contaminant of other strains as same color spore can be produced by a number of fungal strains.
You have to make sure the differences in color are not due to differences in maturation.
Single spore isolation should be performed under a stereomicroscope in order to make sure only one spore is transferred to a fresh medium.
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