I have MC3T3 and RAW cells going for about 2 months now (passage ~20) they still look good but I'm not sure how long can I keep splitting them and using them for experiments or when would be a good time to thaw a new batch.
Hi Aura,
I usually go to passage #30 before thawing a fresh stock.
Kerry
I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.
25 January 2021 3,972 7 View
Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...
14 January 2021 2,316 4 View
27 July 2020 1,141 2 View
I'm conducting a research study titled 'Perception on the business failure prediction using the annual report' to receive the degree of M.Sc. Accounting and Finance by Griffith College in Dublin,...
21 July 2020 8,761 3 View
17 April 2020 5,969 5 View
I have a script that download multiples files from a server, but for each one it requires me to write a password. These are 1000, so it would be laborious to write 1000 times the password. How...
02 April 2020 852 2 View
Hi, everyone. What's the different, if any, between calculating the coefficient of variation of a group of data disposed in a experimental design with different treatments (for ANOVA) and a "free"...
20 March 2020 4,871 3 View
Some of the factors for this to be possible would be: Complete secondary education for girls (and boys); delay the age of marriages to 18 years; women's economic autonomy and access to skills...
11 March 2020 3,837 3 View
I want to remove whole soft tissues and maintain intact the exoeskeleton
10 March 2020 4,345 6 View
I'm looking for .fit images of asteroids (my main interest are the type C, D and P)
08 March 2020 6,862 1 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
02 March 2021 3,060 3 View
NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...
01 March 2021 1,189 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View