Aura María Ramírez

2 Questions 1 Answers 0 Followers

Questions related from Aura María Ramírez

Spontaneous spectinomycin resistance?

I have a mutation that is marked with spectinomycin resistance, I am transducing it into a different background and I am getting colonies (~10), in my no phage control. This has happened with 2...

26 May 2020 1,867 0 View

How long can you keep MC3T3 and Raw cells (osteoblasts and osteoclasts) going?i.e. how many passages before your experiments are no longer comparable?

I have MC3T3 and RAW cells going for about 2 months now (passage ~20) they still look good but I'm not sure how long can I keep splitting them and using them for experiments or when would be a...

10 March 2017 5,419 1 View