How long a protein can be stored?

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How to grow a difficult breast cancer cell line?

Here I am trying to optimizing the growth of breast cancer cell which is ++- requiring additional growth factors (20% FBS, insulin and EGF) for its growth. After adding the required growth factors...

07 August 2019 585 5 View

Any criteria to compare LC/MS/MS query with genome database?

Hi everyone, I am working on a medicinal plant protein belonging to the Brassica family and I have run LC/MS/MS of tryptic peptides digested in solution and trying to find out the results...

05 June 2018 818 1 View

How to interpret ESI spectrum?

Can anyone guide me how to interpret ESI spectrum of plant protein?

04 May 2018 1,932 0 View

LC/ESI/MS program for tryptic in solution digest?

Can anyone provide me the proper program for LC/ESI/MS for tryptic digest in solution. I have literature surveyed and found different suggestions. In some cases, 100 % acetonitrile 0-40 % for...

04 May 2018 8,590 2 View

Fixation of acidic dyes?

How to improve the fixation of acidic dyes on basic fabric? I have tried salts like sodium sulphate but of no use. The dye washes out during washing with cold and hot water. How to improve its...

01 February 2018 3,727 2 View

Tyrosine Standard Curve?

Someone please help me in plotting standard curve for Tyrosine. I made different concentration of tyrosine including 0.05-0.3 mg/ml by dissolving in HCl. Then using 200 microlitre of different...

11 December 2017 6,295 3 View

Relative activity of enzyme?

Please anyone tell me how to calculate the relative activity of amylase, protease and lipase from absorbance? Thank you

11 December 2017 1,633 17 View

To solve CD Spectra?

I got CD spectra of plant protein that is quite similar to that of myoglobin pattern in CD spectrometry. Is that correct spectra? Kindly help me? I am attaching the file below. Thank you

10 November 2017 9,087 2 View

Latest research in Material Science ?

Can any body suggest me a latest topic for research in material science? so that I utilize my time which will be worth full for me in future.

01 February 2017 6,268 0 View

Purification of low molecular weight proteins?

I want to purify low molecular weight proteins particularly lectins from plant. Can anyone give me a suitable protocol to purify lectins from plants? Thank you

08 September 2016 2,121 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

How can CHARMM-GUI be used for protein-ligand interaction ?

My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.

24 June 2024 5,044 1 View

How to predict Protein structure and choose N or C terminal tagging?

Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...

24 June 2024 8,622 6 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Integral Membrane Protein Purification Help?

Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...

30 May 2024 7,604 3 View