Can anyone guide me how to interpret ESI spectrum of plant protein?
Here I am trying to optimizing the growth of breast cancer cell which is ++- requiring additional growth factors (20% FBS, insulin and EGF) for its growth. After adding the required growth factors...
07 August 2019 585 5 View
Hi everyone, I am working on a medicinal plant protein belonging to the Brassica family and I have run LC/MS/MS of tryptic peptides digested in solution and trying to find out the results...
05 June 2018 818 1 View
Can anyone provide me the proper program for LC/ESI/MS for tryptic digest in solution. I have literature surveyed and found different suggestions. In some cases, 100 % acetonitrile 0-40 % for...
04 May 2018 8,590 2 View
How to improve the fixation of acidic dyes on basic fabric? I have tried salts like sodium sulphate but of no use. The dye washes out during washing with cold and hot water. How to improve its...
01 February 2018 3,727 2 View
Someone please help me in plotting standard curve for Tyrosine. I made different concentration of tyrosine including 0.05-0.3 mg/ml by dissolving in HCl. Then using 200 microlitre of different...
11 December 2017 6,295 3 View
Please anyone tell me how to calculate the relative activity of amylase, protease and lipase from absorbance? Thank you
11 December 2017 1,633 17 View
I got CD spectra of plant protein that is quite similar to that of myoglobin pattern in CD spectrometry. Is that correct spectra? Kindly help me? I am attaching the file below. Thank you
10 November 2017 9,087 2 View
Can any body suggest me a latest topic for research in material science? so that I utilize my time which will be worth full for me in future.
01 February 2017 6,268 0 View
I want to purify low molecular weight proteins particularly lectins from plant. Can anyone give me a suitable protocol to purify lectins from plants? Thank you
08 September 2016 2,121 4 View
I found white particles or sample precipitates in the sephadex gel already used 4-5 times. How can I remove it. I have read to use 0.2 M NaOH or non-ionic detergent for cleaning. But I couldn't...
31 December 2015 650 5 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,179 1 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
How can I interpret the data gathered without solving?
03 August 2024 9,054 3 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....
30 July 2024 5,695 0 View
Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.
29 July 2024 4,971 1 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...
23 July 2024 2,269 1 View
