17 Questions 29 Answers 0 Followers
Questions related from Sobia Ejaz
Here I am trying to optimizing the growth of breast cancer cell which is ++- requiring additional growth factors (20% FBS, insulin and EGF) for its growth. After adding the required growth factors...
08 August 2019 558 5 View
Hi everyone, I am working on a medicinal plant protein belonging to the Brassica family and I have run LC/MS/MS of tryptic peptides digested in solution and trying to find out the results...
06 June 2018 798 1 View
Can anyone guide me how to interpret ESI spectrum of plant protein?
05 May 2018 1,907 0 View
Can anyone provide me the proper program for LC/ESI/MS for tryptic digest in solution. I have literature surveyed and found different suggestions. In some cases, 100 % acetonitrile 0-40 % for...
05 May 2018 8,576 2 View
How to improve the fixation of acidic dyes on basic fabric? I have tried salts like sodium sulphate but of no use. The dye washes out during washing with cold and hot water. How to improve its...
02 February 2018 3,700 2 View
Someone please help me in plotting standard curve for Tyrosine. I made different concentration of tyrosine including 0.05-0.3 mg/ml by dissolving in HCl. Then using 200 microlitre of different...
12 December 2017 6,242 3 View
Please anyone tell me how to calculate the relative activity of amylase, protease and lipase from absorbance? Thank you
12 December 2017 1,606 17 View
I got CD spectra of plant protein that is quite similar to that of myoglobin pattern in CD spectrometry. Is that correct spectra? Kindly help me? I am attaching the file below. Thank you
11 November 2017 9,070 2 View
Can any body suggest me a latest topic for research in material science? so that I utilize my time which will be worth full for me in future.
02 February 2017 6,245 0 View
I want to purify low molecular weight proteins particularly lectins from plant. Can anyone give me a suitable protocol to purify lectins from plants? Thank you
09 September 2016 2,101 4 View
I found white particles or sample precipitates in the sephadex gel already used 4-5 times. How can I remove it. I have read to use 0.2 M NaOH or non-ionic detergent for cleaning. But I couldn't...
01 January 2016 628 5 View
If I want to store a plant seed protein, How can I store it and How long can I store it?
10 October 2015 7,460 2 View
I accidently added double volume of bisacrylamide i.e: 1.6 instead of 0.8 during the preparation of solution. Will it effect the sds page or protein bands? Please suggest me. Thank You..........
03 March 2015 5,108 13 View
Can anyone tell me the easiest way to read the mass spectrums for proteins? Also recommend me books or websites relevant to it. Thank you.
01 January 2015 6,999 2 View
I ran gelatin zymography of my plant protein sample but there was no zone of hydrolysis. Moreover, the bands appeared similar as that of sds page bands. I ran sds as well as native zymography and...
01 January 2015 1,902 11 View
Initially when I ran sds-page of my protein sample It gave many bands (around 10 bands in a lane) but now when I did SDS of the same sample it gave fewer bands than before (3-4 bands). Why is it...
12 December 2014 4,158 11 View
I ran a zymogram of protein samples but couldn't get the result. The gel was half stained and half destained showing no zone of hydrolysis. Why is it so? The protein zymogram was run but the gel...
11 November 2014 5,279 19 View
