How to grow a difficult breast cancer cell line?

Here I am trying to optimizing the growth of breast cancer cell which is ++- requiring additional growth factors (20% FBS, insulin and EGF) for its growth. After adding the required growth factors...

07 August 2019 495 5 View

Any criteria to compare LC/MS/MS query with genome database?

Hi everyone, I am working on a medicinal plant protein belonging to the Brassica family and I have run LC/MS/MS of tryptic peptides digested in solution and trying to find out the results...

05 June 2018 744 1 View

How to interpret ESI spectrum?

Can anyone guide me how to interpret ESI spectrum of plant protein?

04 May 2018 1,857 0 View

LC/ESI/MS program for tryptic in solution digest?

Can anyone provide me the proper program for LC/ESI/MS for tryptic digest in solution. I have literature surveyed and found different suggestions. In some cases, 100 % acetonitrile 0-40 % for...

04 May 2018 8,522 2 View

Fixation of acidic dyes?

How to improve the fixation of acidic dyes on basic fabric? I have tried salts like sodium sulphate but of no use. The dye washes out during washing with cold and hot water. How to improve its...

01 February 2018 3,653 2 View

Tyrosine Standard Curve?

Someone please help me in plotting standard curve for Tyrosine. I made different concentration of tyrosine including 0.05-0.3 mg/ml by dissolving in HCl. Then using 200 microlitre of different...

11 December 2017 6,142 3 View

Relative activity of enzyme?

Please anyone tell me how to calculate the relative activity of amylase, protease and lipase from absorbance? Thank you

11 December 2017 1,539 17 View

To solve CD Spectra?

I got CD spectra of plant protein that is quite similar to that of myoglobin pattern in CD spectrometry. Is that correct spectra? Kindly help me? I am attaching the file below. Thank you

10 November 2017 9,030 2 View

Purification of low molecular weight proteins?

08 September 2016 2,048 4 View

How can I clean SEPHADEX gel?

I found white particles or sample precipitates in the sephadex gel already used 4-5 times. How can I remove it. I have read to use 0.2 M NaOH or non-ionic detergent for cleaning. But I couldn't...

31 December 2015 572 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View