In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Can anyone recommend me a valid primer sequences of Human Catalase (CAT) for Real Time PCR (SYBR Green)?

Hi all! I'm planning a Real Time PCR (SYBR Green) to to determine the amount of expression of Human Catalase (CAT) in human cardiomyocyte. I compared some primers found in the literature but none...

19 January 2021 2,233 5 View

What is the blank for catalase activity measurement by spectrophotometer?

I extracted the enzymes of leaves with a potassium phosphate buffer containing PVP and EDTA for catalase activity measurement. The reaction mixture is 3 ml (2.95 ml phosphate buffer+ 0.05 ml...

26 December 2020 606 3 View

Can I dissolve freeze-dried catalase and lysozyme in de-ionised water or do I need a buffer?

Can I dissolve freeze-dried catalase or lysozyme directly into de-ionised water or do I need to use a buffer (separate solutions, not both proteins in one)? I will not be storing the solutions and...

10 November 2020 3,232 5 View

Any article about relation between Catalase inhibition and cell death ?

I am looking for publication involving both a decreased catalase expression and the rate of cell death associated.

08 October 2020 6,373 4 View

Could the hepatic catalase activity increases when we treat mice with immunostimulants?

hello, I would like to know please, if there are cases in which we can observe an increase of catalase activity in mice treated with a therapeutic dose of immunostimulants? I did twice the...

28 September 2020 4,193 3 View

One way ANOVA followed by Dunnett's post hoc test?

I am doing a research in biotechnology where I had my animal studies results. The SD values of the body weight of the animals were conducted. I need to find out the statistical significant values...

23 September 2020 8,722 8 View

Bacteriocin producing LAB?

Hello Dear scientists, I am doing Bacteriocin related research, I did a lot of activities to isolates Bacteriocin producing LAB and I used NaOH, catalase( Sodium thiosulfate as catalase)...

22 August 2020 3,097 3 View