Can I use the calibration equation which was generated using the salt form of a compound, to detect the base form of compound ?

20 February 2016 5,495 1 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Can anyone recommend me a valid primer sequences of Human Catalase (CAT) for Real Time PCR (SYBR Green)?

Hi all! I'm planning a Real Time PCR (SYBR Green) to to determine the amount of expression of Human Catalase (CAT) in human cardiomyocyte. I compared some primers found in the literature but none...

19 January 2021 2,233 5 View

What is the blank for catalase activity measurement by spectrophotometer?

I extracted the enzymes of leaves with a potassium phosphate buffer containing PVP and EDTA for catalase activity measurement. The reaction mixture is 3 ml (2.95 ml phosphate buffer+ 0.05 ml...

26 December 2020 606 3 View

Problem with GST-fusion protein elution- Can anyone help?

18 December 2020 4,559 6 View

What could be the cause of the Glutathione peak migration (LC Dionex, Kintetex EVO C18, water phase) ?

Hello, I need a help from those of you who work on Kinetex EVO C18 with water + 0.2% TFA mobile phase (isocratic). The conditions are constant (temperatures, flow, column, conditioning time)....

16 December 2020 4,582 3 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

10 December 2020 2,198 3 View

Infected plant protein lysate washing out purified GST-tagged protein on agarose resin?

Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...

23 November 2020 7,009 3 View