The question us regarding catalase activity check of bacterial strains. I saw an article in which it was written that we can use 30%hydrogen peroxide for catalase activity but in our laboratories while we actually check catalase activity we don't use 30% hydrogen peroxide, as we believe that it could kill the microorganism. That's why I wanted to be sure that can we use this much percentage and if we can then what should be the other environmental conditions as well as what kind of microorganisms can bear this and why don't we use this percentage usually?
Catalase will be deactivated by excess H2O2. I don`t think it will be active for more than a few seconds with 30% H2O2. Cells will be killed rapidly.
So, it may be useful qualitatively in the sense that you will see oxygen bubbles rapidly, but I don´t think it will give quantitative information because of rapid inactivation of catalase.
Matias Moller Thanks sir, but it would be more helpful if you could recommend me any research article by which I can further distinguish the difference.
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