Bacteria isolated from soybean leaves have characteristic spots. The bacteria are in the form of bacilli, gram negative, positive catalase test, positive oxidase test, and the color of the colony is as shown in the picture. Bacterial colonies are shiny, slimy, convex and raised.
Bacteria isolated on NA medium showed a yellow and slightly orange color. This bacteria was also isolated in YDCA medium and showed a cream color.
I'm looking for pathogenic bacteria that cause disease in soybean plants. Please, help me. Thank you.
Setya Dwi Rahmawati Please, confirm that the bacteria are plant pathogenic. HR test on tobacco and test for soft rot of potato would be useful. Several features that you listed are not sufficient to guess the genus. Look at pages 23-25 in the Introduction to Practical Phytobacteriology A Manual for Phytobacteriology by SAFRINET, the Southern African (SADC) LOOP of BioNET-INTERNATIONAL Compiled by T. Goszczynska, J.J. Serfontein & S. Serfontein. ARC – Plant Protection Research Institute Pretoria, South Africa. 2000. There are some other useful simple tests that may help.
it cannot be correct by these information but it can be Pseudomonas according to Morphology.
Plating out technique could be improved: the objective is to achieve single colonies and to show up contamination. Can't tell much from the info but it looks like a Pseudomonas
The plate dated 12/02/24 looks like a mix of two cultures. It is very important to show single colonies grown on King's B agar (if they are Pseudomonas). Catalase/oxydase positive Pseudomonas are not usual plant pathogens.
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