14 January 2024 1 9K Report

Catalase assay for bacteria - not screening test with hydrogen peroxide

The complete procedure using spectrophotometer

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Why is there no peak shift in FTIR-ATR after the amine-modified polystyrene nanoplastics (PS-NH2) adsorption onto the NIPAAM/CMC hydrogel materials? I have repeated the tests, but I have gotten...

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Why am I observing a m/z peak at 372 for the ciprofloxacin hydrochloride hydrate spectra in MS?

The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.

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What is the significance of -ve 'b' value or -ve intercept for the linear Langmuir isotherm plot??

What is the significance of the -ve 'b' value or -ve intercept for the linear Langmuir isotherm plot?? I recently plotted some of my experimental data in a Langmuir linear isotherm plot. I have...

18 May 2024 4,576 10 View

In Electrostatic Force Microscopy, how Phase (degree) is related with Capacitance?

In EFM measurement, we get topography and Phase image. From phase image what we can conclude? Let say, with thickness of a nanosheet phase is increasing. What does it mean that with thickness...

08 May 2024 7,590 1 View

How can I get involved in writing a chapter of scientific book in my research area?

Any lead will be helpful

24 April 2024 5,987 3 View

Why does IR spectrum of a compound of mine show a peak more than 100% transmittance??

The instrument provided data of the IR spectrum is in %T vs wave number. But the peak is in the 3600 to 2600 cm-1 shows more than 100% transmittance. What are the probable reasons behind it? How...

21 April 2024 1,745 1 View

HeLa cells have been detaching after heat stress. Can someone please suggest some way to keep the cells adhered during heat stress and recovery?

I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...

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How can I measure the zeta potential of hydrogel beads?

Actually, the Zeta potential measurement instrument (Zeta Sizer, DLS method) needs the refractive index and absorbance values of the hydrogel beads. How can I measure these two parameter values...

28 March 2024 6,947 4 View

Hi, can anyone suggest anti-ubiquitin antibody that is specific for mono-ubiquitination and does detect not poly-ubiquitination or free Ubiquitin?

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19 November 2023 7,374 0 View

Why does Metaboanalyst 5.0 analyze up to 5000 features only? On what basis it choses 5000 features from a large dataset (eg. 15000 features)?

Recently, I am using Metaboanalyst 5.0 to find out differentially expressed metabolites. I have over 15000 features in my dataset. However, after successfully uploading the data in Metaboanalyst...

09 November 2023 4,347 0 View

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