14 January 2024 1 9K Report

Catalase assay for bacteria - not screening test with hydrogen peroxide

The complete procedure using spectrophotometer

More Subhoshmita Mondal's questions See All
How to perform Gaussian optimization for a polymeric structure?

I am trying to perform Gaussian optimization of a polymeric structure with 250 atoms. It is made up of only C and H atoms with several aromatic phenyl ring interlinked by C=C. Every time I am...

04 January 2021 9,616 2 View

Can I calculate discharge of streams/ watersheds in GIS without field data?

I am required to do a project where I have to calculate discharge of streams/ watersheds in a high altitude area with extremely treacherous terrain. Will it be possible to achieve this objective...

27 December 2020 9,101 5 View

If one publishes in a journal with impact factor >1.4 and which is not included in NAAS list of journals, will the paper be equally valued by ICAR?

I understand the difference between NAAS score and Impact Factor but is it okay to publish in a non-NAAS, high IF journal? How will it be valued in ICAR interviews and related recruitments?

10 September 2020 7,575 3 View

How can I perform sensitivity analysis using R?

I have below model: X = A*B Where A = a list of values with Lognormal distribution (size = 13) and B = another list of values with Lognormal distribution (size = 13) How can I perform 1st...

05 July 2020 1,963 1 View

We have some proteomics data from 2017 and 2018 for which the raw files are missing, but we have the processed raw data. Is the data publishable?

We are missing some mass-spec raw files from 2017 and 2018 when deposition of raw files were not mandatory. Since most of the journals now require raw file availability through ProteomeXchange,...

14 June 2020 6,064 1 View

Why second stage juveniles are used for DNA extraction from nematode for identification purpose? Can I use female or male for the same purpose?

I am working on rice root knot nematode. For identification purpose mostly second stage juveniles are used. I wonder why females or males are not used commonly for the same purpose? I am using...

26 March 2020 7,419 3 View

Is there any alternative cheaper TaqMan universal master mix available in the market other than Thermo with similar sensitivity?

I am looking for a cheaper TagMan master mix for qPCR. I use FAM labeled primers for gene expression, does anyone have any suggestions

17 March 2020 1,105 2 View

What is the effect of Bejan number on heat transfer applications?

In our case the Bejan number decreases with the increase of mass flow rate or Reynolds number of the multiphase fluids through a packed bed. However, if mass flow rate increases Nusselt number,...

27 December 2019 6,088 3 View

Is range of the induced vector measure of a Riemann-Pettis integrable function relatively compact in X?

A function $f\in X^{[a, b]}$ is said to be Riemann-Pettis integrable if $x^* f$ is Riemann integrable for each $x^*\in X^*$ and if for each interval $I\subset [a, b]$, there is a vector in $x_I\in...

27 December 2019 7,532 9 View

Higher or lower values of local entropy generation rate is desirable for the better heat transport?

Local entropy generation rate (comes from the 2nd law of thermodynamics) should be higher or lower for a heat transfer device for its better heat transport performance?

22 December 2019 5,338 3 View

Similar questions and discussions
How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close