How far away should the mature miRNA be from the head of the precursor ‘stem? Can the mature miRNA be a part of the head? Any references or pictures?

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I tried to germinate foxtail seeds but did not work even after vernalization and GA treatment. Any help? Is there any standard germination protocol?

I tried to germinate foxtail seeds but did not work even after vernalization and GA treatment. Any help? Is there any standard protocol to grow this plant?

04 May 2018 3,318 4 View

Publish your research at Frontiers Journal in Plant Science (IF=3.6)

Are you are interested in publishing an article related to epigenetics, evolution and stress in plants in "Frontiers Journal in Plant Science"?If you are interested please look at the following...

08 September 2014 2,478 0 View

Is there an IF for Methods in molecular biology?

Is there an impact factor (IF) for Methods in molecular biology. I see ResearchGate adding an IF for it. This Journal (book chapter) is available through the Medline database.

08 September 2014 7,005 0 View

Is there a web-based software available to name newly discovered and non conserved miRNA based on the deviation from the original miRNA sequence?

05 June 2014 748 3 View

I am looking for a web based program for gene annotation and make gene ontology study. Can anyone help?

I would like to functionally annotate a group of genes. I am having difficulties using Blast 2 go.

04 May 2014 8,460 5 View

Is there a software that can compare two phylogenetic trees to each othe (face to face comparison)?

I want to compare the results I obtained using two different phylogenetic trees. I would like to have some lines between the species in the two trees.The phylogenetic trees or the dendograms are...

03 April 2014 3,678 16 View

Is there a universal primer kit which is comercially available for all known miRNA for RT-PCR?

03 April 2014 1,348 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Some new emerging problems on application of RL for scheduling in IoT networks?

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01 August 2024 8,754 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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30 July 2024 6,420 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View

Anurag Chaturvedi

Check out this page:

http://www.nature.com/reviews/focus/microrna/index.html

http://bib.oxfordjournals.org/content/early/2012/11/22/bib.bbs075.full

Basically it contains some reviews of miRNA.

Sung-Chou Li

You may refer to the bellowing reference:

Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15565168