I tried to germinate foxtail seeds but did not work even after vernalization and GA treatment. Any help? Is there any standard germination protocol?

I tried to germinate foxtail seeds but did not work even after vernalization and GA treatment. Any help? Is there any standard protocol to grow this plant?

04 May 2018 3,216 4 View

Is there an IF for Methods in molecular biology?

08 September 2014 6,908 0 View

Publish your research at Frontiers Journal in Plant Science (IF=3.6)

Are you are interested in publishing an article related to epigenetics, evolution and stress in plants in "Frontiers Journal in Plant Science"?If you are interested please look at the following...

08 September 2014 2,336 0 View

Is there a web-based software available to name newly discovered and non conserved miRNA based on the deviation from the original miRNA sequence?

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05 June 2014 659 3 View

I am looking for a web based program for gene annotation and make gene ontology study. Can anyone help?

I would like to functionally annotate a group of genes. I am having difficulties using Blast 2 go.

04 May 2014 8,366 5 View

Is there a software that can compare two phylogenetic trees to each othe (face to face comparison)?

I want to compare the results I obtained using two different phylogenetic trees. I would like to have some lines between the species in the two trees.The phylogenetic trees or the dendograms are...

03 April 2014 3,585 16 View

Is there a universal primer kit which is comercially available for all known miRNA for RT-PCR?

Is there a universal primer kit which is comercially available for all known miRNA for RT-PCR?

03 April 2014 1,248 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View