How do I see focus/foci formation in breast cancer cell lines -MCF 7 or MDA MB 231?

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Why different plasmid with same back bone shows difference in mammalian (HEK293T) transfection efficiency with same transfection agent?

Hi All. I am using 4 different plasmid, each encoding different scFv with similar back bone, The only difference between all the plasmid is nucleotide encoding for scFv seq (Antibody Seq)....

01 February 2019 2,036 12 View

Is it advisable to calculate IC50 when my drug concentration haven't reached IC:50 ?

08 September 2016 8,596 4 View

Why body of tumor fails to carry single mutation that first arise/ initiated in stem cell ?

We all know that CSC (Cancer stem cell) are germ line of tumor giving rise to body of tumor cell. Then why this body of tumor cell fails to carry single gene mutation or single gene alteration,...

05 June 2016 9,112 3 View

Which type of cell is more likely to become tumor ? in the contest of Doubling time and Life span ?

1) Which one of the following has increased risk of becoming tumor ? A) Cells with shortest doubling time (often divide) and shortest life span. B) Cells with longest doubling time (rarely divide)...

05 June 2016 4,577 1 View

Is there any gene specific carcinogen ?

05 June 2016 5,537 4 View

What happen when suspension of malignant tumor is auto-grafted to the patient from whom tumor is isolated? will it form tumor mass ?

05 June 2016 1,817 6 View

Can cancer call die without morphological changes ? or Dead cancer cell can continue to have intact morphology ? Will it be called senescence

03 April 2016 4,243 10 View

Cancer or tumor can arise from normal cell without any genetic changes ?

Here You go .. I was looking for answer ,can tumor arise from normal cell without genetic or epigenetic changes .. Answer is YES .. Here you Go...

06 July 2015 9,421 0 View

How is stacking is formed on the DNA ladder assay when the genome of a cell is cleaved uniformly into 180 bp during apoptosis?

As we know well the genomic DNA is cleaved into nucleosomal sub units by CAD enzyme during Apoptosis .. If every part of genomic DNA is cleaved to 180bp (Nucleosomal sub unit) ,then how do we get...

08 September 2014 6,869 5 View

How do MAMP can make the immune system to discriminate between beneficial micro organism and pathogen ?

06 July 2014 5,706 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View