Suresh Oncosuresh

16 Questions 29 Answers 0 Followers

Questions related from Suresh Oncosuresh

Why different plasmid with same back bone shows difference in mammalian (HEK293T) transfection efficiency with same transfection agent?

Hi All. I am using 4 different plasmid, each encoding different scFv with similar back bone, The only difference between all the plasmid is nucleotide encoding for scFv seq (Antibody Seq)....

02 February 2019 2,126 12 View

Is it advisable to calculate IC50 when my drug concentration haven't reached IC:50 ?

Here is an example : concentration Average Percentage viability in normal cell line 0.1 1001 98.2310 ...

09 September 2016 8,668 4 View

Which type of cell is more likely to become tumor ? in the contest of Doubling time and Life span ?

1) Which one of the following has increased risk of becoming tumor ? A) Cells with shortest doubling time (often divide) and shortest life span. B) Cells with longest doubling time (rarely divide)...

06 June 2016 4,662 1 View

Is there any gene specific carcinogen ?

if there is any gene specific carcinogen (che,bio,phy),we can initiate gene alteration specifically to tumor and later we can develop drug for it ... Carcinogen , Gene alteration, targeted drug

06 June 2016 5,616 4 View

Why body of tumor fails to carry single mutation that first arise/ initiated in stem cell ?

We all know that CSC (Cancer stem cell) are germ line of tumor giving rise to body of tumor cell. Then why this body of tumor cell fails to carry single gene mutation or single gene alteration,...

06 June 2016 9,201 3 View

What happen when suspension of malignant tumor is auto-grafted to the patient from whom tumor is isolated? will it form tumor mass ?

Consider this scenario, where 35 year old female is diagnosed for breast cancer (malignant), her Breast tumor is completely dissected from her breast or body via mastectomy or lumpectomy. Later...

06 June 2016 1,908 6 View

Can cancer call die without morphological changes ? or Dead cancer cell can continue to have intact morphology ? Will it be called senescence

During MTT assay, after seeding we unknowingly forget to cover 96 well plate with the given lid and we incubated for 24hr in 5% Co2, humidified with copper sulphate. After 24 hr of seeding, we...

04 April 2016 4,352 10 View

Cancer or tumor can arise from normal cell without any genetic changes ?

Here You go .. I was looking for answer ,can tumor arise from normal cell without genetic or epigenetic changes .. Answer is YES .. Here you Go...

07 July 2015 9,503 0 View

How is stacking is formed on the DNA ladder assay when the genome of a cell is cleaved uniformly into 180 bp during apoptosis?

As we know well the genomic DNA is cleaved into nucleosomal sub units by CAD enzyme during Apoptosis .. If every part of genomic DNA is cleaved to 180bp (Nucleosomal sub unit) ,then how do we get...

09 September 2014 6,957 5 View

Will ALL acute lymphoblastic leukemia T lymphocyte/B lymphocytes protect themselves from foreign substances (Microbes) in cancer patients?

Will ALL acute lymphoblastic leukemia T lymphocyte/B lymphocytes protect themselves from foreign substances (Microbes) in cancer patients? If Yes, how will they do this? Being immature cells, how...

07 July 2014 3,797 5 View

How do MAMP can make the immune system to discriminate between beneficial micro organism and pathogen ?

Both beneficial and pathogenic organism might have similar MAMP ( microorganism associated molecular pattern) such as flagellin,LPS dsRNA.CpG etc .HOW do our Host cell TLR(Toll like receptor) can...

07 July 2014 5,784 8 View

How do I see focus/foci formation in breast cancer cell lines -MCF 7 or MDA MB 231?

Or any over grown cell line after reaching confluency using inverted microscope? Did anyone see foci in T25 flask with normal inverted microscope? I have read and saw the image in 'Biology of...

09 September 2013 7,314 2 View

Why does cancer cell have to metastasise? Why is it necessary when it can survive in hypoxy condition and self sufficiency growth signal?

We human migrate from one place to another, when we could not able to find the basic needs like water shelter, food. But why cancer cell has to migrate from its place of origin?We know how and...

09 September 2013 3,901 12 View

How inhibiting the activity of a single oncogene and its associated pathway in vitro and or in vivo kills the tumor cell ?

It is well known that Single mutant Proto Oncogene is not sufficient to initiate tumor /bring Biochemical and morphological charecterisitc associted with tumor such as foci formation,invasion etc...

09 September 2012 9,431 0 View

Can a tumor arise from differentiated cell ? (or) Can differentiated cell be reverted to undifferentiated /de differentiated ?

Can a tumor arise from differentiated cell ? (or) Can differentiated cell be reverted to undifferentiated /de differentiated ? Can a malignant be reverted to benign .. Please do let me know...

09 September 2012 5,939 0 View

Why Dendritic cell are not killed by T cells when they present antigen ?

Dendritic cell present antigenic peptide to CD4+Tcell or CTLs and activate them to kill tumor cell or viral infected cell ,which is specific to the antigen presented by Dendritic cell .. My...

10 October 2011 6,402 21 View