Why different plasmid with same back bone shows difference in mammalian (HEK293T) transfection efficiency with same transfection agent?

Hi All. I am using 4 different plasmid, each encoding different scFv with similar back bone, The only difference between all the plasmid is nucleotide encoding for scFv seq (Antibody Seq)....

01 February 2019 2,036 12 View

Is it advisable to calculate IC50 when my drug concentration haven't reached IC:50 ?

08 September 2016 8,596 4 View

Why body of tumor fails to carry single mutation that first arise/ initiated in stem cell ?

We all know that CSC (Cancer stem cell) are germ line of tumor giving rise to body of tumor cell. Then why this body of tumor cell fails to carry single gene mutation or single gene alteration,...

05 June 2016 9,112 3 View

Which type of cell is more likely to become tumor ? in the contest of Doubling time and Life span ?

1) Which one of the following has increased risk of becoming tumor ? A) Cells with shortest doubling time (often divide) and shortest life span. B) Cells with longest doubling time (rarely divide)...

05 June 2016 4,577 1 View

Is there any gene specific carcinogen ?

05 June 2016 5,537 4 View

What happen when suspension of malignant tumor is auto-grafted to the patient from whom tumor is isolated? will it form tumor mass ?

05 June 2016 1,817 6 View

Can cancer call die without morphological changes ? or Dead cancer cell can continue to have intact morphology ? Will it be called senescence

03 April 2016 4,243 10 View

How is stacking is formed on the DNA ladder assay when the genome of a cell is cleaved uniformly into 180 bp during apoptosis?

As we know well the genomic DNA is cleaved into nucleosomal sub units by CAD enzyme during Apoptosis .. If every part of genomic DNA is cleaved to 180bp (Nucleosomal sub unit) ,then how do we get...

08 September 2014 6,869 5 View

How do MAMP can make the immune system to discriminate between beneficial micro organism and pathogen ?

06 July 2014 5,706 8 View

Will ALL acute lymphoblastic leukemia T lymphocyte/B lymphocytes protect themselves from foreign substances (Microbes) in cancer patients?

06 July 2014 3,722 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View