In http://www.sciencedirect.com/science/article/pii/S1383574215000022 the authors review some articles showing that chemo and radiotherapy can introduce new heteroplasmic variation in mtDNA. Is it possible to use for example cisplatin or radiation to obtain a subclone cell line with an easily traceable new pattern of heteroplasmy? I would like to use three cell lines of the same cancer origin but with different patterns of heteroplasmy so it would be possible to distinguish them by mtDNA sequencing. I do not want to use cybrids. Someone did something similar? How many passages would it take obtain a subclone with an easily traceable different pattern of mtDNA heteroplasmy suppose I will have an efficient mutating agent? Is there some chemical mutating agent apart from cisplatin available which is more selective towards mtDNA?