18 November 2014 2 4K Report

We are measuring confluence by TEER, a stained sentinel insert and permeability of FIT-C. I've tried passage 2-10, I've tried 100-400k seeding densities, yet none seem to reach confluence in the inserts. The same cells are fine and reach confluence in 75cm^2 flasks within 3-4 days. Any suggestions would be highly appreciated.

More Aja L O Mason's questions See All
Similar questions and discussions