How can we get our HUVECs from Lonza to reach confluence in 24-well transwell collagen coated PTFE inserts?

We are measuring confluence by TEER, a stained sentinel insert and permeability of FIT-C. I've tried passage 2-10, I've tried 100-400k seeding densities, yet none seem to reach confluence in the...

18 November 2014 4,218 2 View