Is it possible to perform cardiac puncture followed by PBS perfusion with a pump?

Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...

15 February 2021 5,312 2 View

What is the difference between blocking buffer tbs vs. pbs and which should I use?

Hi everybody! I am doing western blots on bovine mammary epithelial cells to determine casein. We have two types of blocking buffer in the lab. PBS:...

01 February 2021 9,278 5 View

What types of tubes should I use for allantoin assay?

Hi everyone, I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:...

07 January 2021 8,023 3 View

What is growing inside a frozen 10X Ripa buffer?

Hello all! I have a strange question today...I noticed that a few of my aliquotted 10x RIPA buffers that were in the -20 C had some kind of potential mold (white balls, some clusters, some with...

20 December 2020 7,424 3 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

What are these called?

Upon tissue plating for cell isolation, I start to see fibroblasts and after a while these 3D structures start to form. Are they called stroma? Someone in my building told me that she calls them...

30 November 2020 8,685 4 View

Plated Secretory Tissue from Udder--What type of cells are these?

Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...

28 October 2020 7,768 3 View

How difficult is Lysosensor to use in the lab?

How difficult is Lysosensor to use in a basic cell culture wet lab? Does anybody happen to have any advice and/or experience with this or similar probes? Thanks in advance!

15 September 2020 8,730 7 View

Quand est ce qu'un chercheur peut-il se sentir satisfait de ses publications?

18 May 2020 7,762 2 View

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18 May 2020 6,271 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View