longevity, solidity, patency, reliability.... Thanks
Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...
15 February 2021 5,312 2 View
Hi everybody! I am doing western blots on bovine mammary epithelial cells to determine casein. We have two types of blocking buffer in the lab. PBS:...
01 February 2021 9,278 5 View
Hi everyone, I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:...
07 January 2021 8,023 3 View
Hello all! I have a strange question today...I noticed that a few of my aliquotted 10x RIPA buffers that were in the -20 C had some kind of potential mold (white balls, some clusters, some with...
20 December 2020 7,424 3 View
Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...
06 December 2020 1,704 3 View
Upon tissue plating for cell isolation, I start to see fibroblasts and after a while these 3D structures start to form. Are they called stroma? Someone in my building told me that she calls them...
30 November 2020 8,685 4 View
Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...
28 October 2020 7,768 3 View
How difficult is Lysosensor to use in a basic cell culture wet lab? Does anybody happen to have any advice and/or experience with this or similar probes? Thanks in advance!
15 September 2020 8,730 7 View
18 May 2020 7,762 2 View
18 May 2020 6,271 2 View
Source to get Impact factor of journals
01 March 2021 6,654 2 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...
26 February 2021 3,404 10 View
For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...
23 February 2021 1,346 13 View
Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...
22 February 2021 8,132 6 View
Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...
21 February 2021 7,457 3 View
We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...
16 February 2021 678 3 View
Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!
15 February 2021 2,874 4 View
There are 6 television programmes of different TV genres and their mean and mean ranks are put for each of them. But the reliability value of all the programmes together is 0.498 . So what exactly...
15 February 2021 7,686 4 View
when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...
14 February 2021 5,778 9 View
