Alexis Broquet

14 Questions 16 Answers 0 Followers

Questions related from Alexis Broquet

How to keep alive isolated murine gdT cells in culture?

Hello everybody, We would like to address the function of our gdT cells in vitro. These cells are isolated by positive selection from murine lungs and we would like to assess there antitumoral...

06 March 2023 6,459 4 View

How to culture PBMC for 7 days?

I'm trying to cultivate PBMC (fresh or frozen) for 7 days and I don't see monocytes (CD14+) anymore at the end of the culture. here is my protocol so far: PBMC fresh: Ficoll and retrieve of the...

20 May 2021 885 5 View

How to get reliable and consistent LLC and B16/F10 lung metastasis count in mice?

Hi, I'm facing 2 issues with our LLC and B16/F10 melanoma model in order to count lung metastasis. In order to get significant metastasis, we need to incubate LLC and B16/F10 in mice for about a...

20 May 2021 4,707 2 View

What is the stability of urethane in PBS solution?

We are starting a urethane-induced lung cancer model in mice (sigma U2500) and are wondering if we need to prepare urethane solution the day of the injection or if urethane solution (100mg/mL) in...

20 May 2021 9,628 0 View

How to get a nice CD107a signal in mouse by flow cytometry?

I tried to monitored CD107a labelling in mouse NK cells after infection (compared to uninfected) but I'm unhappy with the plot I got. CD107a Ab clone 1D4B rat IgG2a,k from BD Bioscience cell are...

10 October 2018 4,966 2 View

Has anyone been successful with anti pSTAT3 Ab (Cell signaling #9145) in paraffin IHC mouse lungs?

Hello, I'm having trouble getting a positive signal using the Cell Signaling Ab anti)phosphor STAT3 (#9145) in a paraffin embedded mouse lung section. Have anyone got a simple and convenient...

10 January 2018 2,225 0 View

Where can I find Fluorescent-tagged bacteria?

For our studies we would like to use fluorescent-tagged Pseudomonas aeruginosa and Staphylococcus aureus in GFP or other dye. Does anybody know where to get these? Or does anybody willing to...

30 April 2015 332 3 View

Are Cytokines sensitive to medium/high temperature (50-60°C)?

Hi, I am wondering whether cytokines such as TNF alpha, IL1 beta,..) are sensitive to temperature values as 50-60°C and might loose their biological activities. I cannot get an answer through...

27 October 2014 9,763 3 View

Which is the best way to deplete alveolar macrophages in mice?

We would like to deplete alveolar macrophages in mice and we cannot find suitable antibodies (F4/80?). We will be using random Swiss mice (non isogenic). The only way I found is by using...

29 July 2014 7,253 2 View

How can we easily isolate mouse lymph node?

We are interested in analysis regulatory T cell pop in a infectious murine model. Has anyone got an easy protocol to get mesenteric lymph node for example. And also what does it look like? I'm...

24 June 2014 6,470 2 View

Mice general shape scoring scale?

Hello all, I'm working in the field of infectious diseases and I'm using mice models of infection. I saw a paper some times ago in which the authors using a scoring system in order to measure the...

24 January 2014 9,034 2 View

Anti-CD11c mouse Ab working in paraffin-embedded sections?

We have been trying to get by IHC labeling of CD11c positive cells in our lungs and spleen sample from mice. The best we could get was a weird labeling in red. We do not label the cells as the...

24 January 2014 8,593 14 View

Does anybody know a working antibody anti F4/80 working in paraffin-embedded mouse tissue?

We have been trying to get by IHC labeling of F4/80 positive cells in our lungs sample from mice (macrophages) The best we could get was a weird labeling in red. We do not label the cells as the...

24 January 2014 9,434 2 View

Has anyone ever used an antibody designed to detect P. aeruginosa (strain PAO10 in parafin-embedded mouse tissues)?

I checked on pubmed and on the biocompare.com website and found no antibodies or related studies so far.

14 December 2012 8,737 3 View