15 Questions 19 Answers 0 Followers
Questions related from Alexis Broquet
Hello everybody, We would like to address the function of our gdT cells in vitro. These cells are isolated by positive selection from murine lungs and we would like to assess there antitumoral...
06 March 2023 6,462 4 View
I'm trying to cultivate PBMC (fresh or frozen) for 7 days and I don't see monocytes (CD14+) anymore at the end of the culture. here is my protocol so far: PBMC fresh: Ficoll and retrieve of the...
20 May 2021 888 5 View
Hi, I'm facing 2 issues with our LLC and B16/F10 melanoma model in order to count lung metastasis. In order to get significant metastasis, we need to incubate LLC and B16/F10 in mice for about a...
20 May 2021 4,710 2 View
We are starting a urethane-induced lung cancer model in mice (sigma U2500) and are wondering if we need to prepare urethane solution the day of the injection or if urethane solution (100mg/mL) in...
20 May 2021 9,633 0 View
I tried to monitored CD107a labelling in mouse NK cells after infection (compared to uninfected) but I'm unhappy with the plot I got. CD107a Ab clone 1D4B rat IgG2a,k from BD Bioscience cell are...
10 October 2018 4,972 2 View
Hello, I'm having trouble getting a positive signal using the Cell Signaling Ab anti)phosphor STAT3 (#9145) in a paraffin embedded mouse lung section. Have anyone got a simple and convenient...
10 January 2018 2,229 0 View
I'm trying to detect a receptor on the surface of my A549 cells. After trypsin treatment, I see no specific labelling although previous published report the expression of my target. One paper...
18 November 2016 7,086 3 View
For our studies we would like to use fluorescent-tagged Pseudomonas aeruginosa and Staphylococcus aureus in GFP or other dye. Does anybody know where to get these? Or does anybody willing to...
30 April 2015 340 3 View
Hi, I am wondering whether cytokines such as TNF alpha, IL1 beta,..) are sensitive to temperature values as 50-60°C and might loose their biological activities. I cannot get an answer through...
27 October 2014 9,766 3 View
We would like to deplete alveolar macrophages in mice and we cannot find suitable antibodies (F4/80?). We will be using random Swiss mice (non isogenic). The only way I found is by using...
29 July 2014 7,257 2 View
We are interested in analysis regulatory T cell pop in a infectious murine model. Has anyone got an easy protocol to get mesenteric lymph node for example. And also what does it look like? I'm...
24 June 2014 6,473 2 View
Hello all, I'm working in the field of infectious diseases and I'm using mice models of infection. I saw a paper some times ago in which the authors using a scoring system in order to measure the...
24 January 2014 9,037 2 View
We have been trying to get by IHC labeling of CD11c positive cells in our lungs and spleen sample from mice. The best we could get was a weird labeling in red. We do not label the cells as the...
24 January 2014 8,602 14 View
We have been trying to get by IHC labeling of F4/80 positive cells in our lungs sample from mice (macrophages) The best we could get was a weird labeling in red. We do not label the cells as the...
24 January 2014 9,436 2 View
I checked on pubmed and on the biocompare.com website and found no antibodies or related studies so far.
14 December 2012 8,740 3 View
