How can we determine the threshold for binary discrimination in pyrosequencing methylation analysis (PMA)?

11 November 2014 1 8K Report

How to determine the threshold for binary discremination in pyrosequencing methylation analysis (PMA)?

As we known, pyrosequencing will refer continuous signal for DNA methylation in a specific region. How can we cut-off it as the binary status including methylation and un-methylation as the table 2 showed in the following paper:

http://www.ncbi.nlm.nih.gov/pubmed/22962272

Biswapriya Biswavas Misra

Have you tried to post the question at SEQ Answers and Biostar forums as well ? Best wishes.

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Science method

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