How the cytotoxicity can be measured for metallic materials? Is it mandatory for a Material Engg./Mech Eng. to have a medical Practitioner with them?

I do not know the much about cyto-toxicity test for metallic or polymer material? Is the test type different for different material? Also if you are a Material Engineer or Mechanical Engineer,...

01 February 2018 1,427 3 View

What is the best method to learn FEM, if we want to incorporate it into our research.

I want to make models of my material for analysis purpose, so please suggest me.

04 May 2017 274 1 View

What is the difference between clock time and CPU time during the Genetic Algorithm Computation?

04 May 2016 2,394 0 View

What is the mechanism that exists behind the working of functional graded materials?

The deformation response of material depends upon the elastic modulus of its constituents, so with increase in grading parameter,the ceramic volume increases and because as elastic modulus of...

10 November 2015 5,948 14 View

What is the Best method to solve optimization problems?

Now a day, a lot of optimization methods are available, like Taguchi (Grey relational, utility concept, Entropy Method), RSM (Desirability), GA (NSGA-II), ANN, PSO, Ant colony optimization...

10 November 2015 9,895 4 View

How we can analyse the XRD pattern?

Can anybody help me to study XRD pattern? I had two graph, but unable to explain.

11 December 2014 6,190 5 View

What part of MEMS relate to Electronics and how can we combine both?

Micro electro-mechanical system consist both mechanical and electronics part. so how can we combine both. what part is of electronics and how can be combine with mechanical?

10 November 2014 181 2 View

What is the minimum size of tensile test specimen according to ASTM?

I want to fabricate one dye for powder compaction and samples for tensile test. What is the minimum size of tensile test specimen so that a smaller dye can be made according to specimen?

03 April 2014 9,718 23 View

How can we combine Taguchi Technique with GA?

I want to design the experiments from Taguchi design. Where can I get a model which can be processed from GA?

03 April 2014 7,377 8 View

How can I measure the shape memory effect of an alloy?

Methods of Measurement of SME of an alloy

02 March 2014 2,516 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View