It really varies from cell line to cell line.

Sometimes, you can use the EDTA without the trypsin (called Versene). Sometimes piptting the media vigorously on the cells can also dislodge them.

It really varies from cell line to cell line.

Sometimes, you can use the EDTA without the trypsin (called Versene). Sometimes piptting the media vigorously on the cells can also dislodge them.

also i have used EDTA for this, it will depend on the cell type too,

I used at 1-2 mM EDTA/PBS.

https://www.researchgate.net/post/Trypsin-Free_Detachment_of_cells_using_EDTA

using cell scrapers lift the mono-layer is another option. 

https://biology.stackexchange.com/questions/23253/alternatives-to-trypsin-for-cell-detachment

Best Wishes,

you can try cell scraping but it might cause damage to cells. 

Hi Javier,

We had the same requirement for our adhesion studies where we needed the surface proteins intact and so couldn't use trypisn.

We used the Cell Dissociation solution Buffer (C5914 SIGMA)-  it made a big difference in our results..  Hope it works for you..

All the best!

Thaks Suja,

Best.