Javier Benjamín Balam Romero

7 Questions 13 Answers 0 Followers

Questions related from Javier Benjamín Balam Romero

Has anyone had experience transfecting human macrophages with the plasmid pEGFP-N1?

I have a problem. I need to transfect macrophages derived form PBMC with the plasmid pEGFP-N1 using Lipofectamine LTX. I have used the protocol suggested by the commercial house, but I still can...

04 April 2018 8,916 1 View

How can I take off cells from a monolayer without destroying their proteins?

I will do a cell culture, but I can not use trypsin-EDTA because it will lysate the membrane proteins and I'm interested in quantifying those proteins. Is there another way to take off the cells...

09 September 2017 1,479 6 View

I need help with the propagation of BCG (Mycobacterium bovis). Does anyone know which culture conditions need?

Propagation of BCG

09 September 2013 5,805 6 View

Differences between IL-12 p40 and IL-12 p70?

I wish to quantify cytokines in supernatant obtained lymphoproliferation assays but first I want to select which IL-12 should be tested.

09 September 2013 6,391 13 View

Has anyone used Lipofectamine LTX + Plus Reagent for transfection in macrophages from THP-1 cells?

Tell me about your experiences in transfection please, thanks!

08 August 2013 9,867 3 View

How can Lymphocytes cells for transportation be preserved?

I need to transport cells from one country to another for further studies but is essential to preserve the viability.

08 August 2013 6,747 5 View

Fetal bovine serum can cause false positives in assays for human lymphocyte proliferation?

I'm doing a proliferation assay of human lymphocytes, but I would like to know if FBS may induce proliferation in controls. I´ll use RPMI-1640 medium to assay with FBS but, I think this serum can...

08 August 2013 9,557 13 View