Dear Javier..
You can grow BCG in 7H9 broth as well as in 7H10 agar with 10% OADC as growth supplement. You can incubate the cultures at 37 degree Celsius. Sometimes it is also recommended to keep cultures in 37 degree Celsius along with 5% C02. As M.bovis is a slow growing bacterium it will take a month to form a visible colony in the 7H10 agar plate and almost 20-25 days in 7H9 broth.
Dear Javier..
You can grow BCG in 7H9 broth as well as in 7H10 agar with 10% OADC as growth supplement. You can incubate the cultures at 37 degree Celsius. Sometimes it is also recommended to keep cultures in 37 degree Celsius along with 5% C02. As M.bovis is a slow growing bacterium it will take a month to form a visible colony in the 7H10 agar plate and almost 20-25 days in 7H9 broth.
Dear Javier,
you can:
-use Löwenstein-Jensen slants and incubate at 37ºC and 25ºC for 8 weeks
( http://www.scielo.br/scielo.php?pid=S1517-83822004000100004&script=sci_arttext )
- materials&methods: http://jcm.asm.org/content/49/3/802.full
-maybe this is interesting too: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2002.03237.x/full
Julien Wawrzyniak, did you move forward with flow cytometry to estimate growth? I'm asking out of curiosity because we are discussing this option in a project that I just got involved in. So I would be very interested to hear about your experience wth this.
Cheers, Mille
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