Has anyone used Lipofectamine LTX + Plus Reagent for transfection in macrophages from THP-1 cells?

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Kezhuo Zhang

Hi. Sorry I don't have an answer for your questions. But I would like to know how it works for you. Recently we are trying to use LTX or LTX-Plus for Jurkat cells transfection. Or do you have a better transfection kit for this hard-transfected cell?

Thanks a lot.

Javier Benjamín Balam Romero

Dear Zhang.

I´m using LTX with this cell line, but I don´t have transfected cell, for this reasons I wrote my cuestion. Sorry.

Best!

Raja Veerapandian

Kindly tell me whether you have succeeded in transfection or what type of difficulties you have came across and also tell me the protocol which you have followed. Transfection protocol of suspension cells will differ from adherent cells.