Has anyone had experience transfecting human macrophages with the plasmid pEGFP-N1?

I have a problem. I need to transfect macrophages derived form PBMC with the plasmid pEGFP-N1 using Lipofectamine LTX. I have used the protocol suggested by the commercial house, but I still can...

03 April 2018 8,866 1 View

How can I take off cells from a monolayer without destroying their proteins?

I will do a cell culture, but I can not use trypsin-EDTA because it will lysate the membrane proteins and I'm interested in quantifying those proteins. Is there another way to take off the cells...

08 September 2017 1,459 6 View

I need help with the propagation of BCG (Mycobacterium bovis). Does anyone know which culture conditions need?

Propagation of BCG

08 September 2013 5,770 6 View

Differences between IL-12 p40 and IL-12 p70?

I wish to quantify cytokines in supernatant obtained lymphoproliferation assays but first I want to select which IL-12 should be tested.

08 September 2013 6,365 13 View

Fetal bovine serum can cause false positives in assays for human lymphocyte proliferation?

07 August 2013 9,530 13 View

How can Lymphocytes cells for transportation be preserved?

I need to transport cells from one country to another for further studies but is essential to preserve the viability.

07 August 2013 6,727 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

01 March 2021 3,905 5 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View