How can I take off cells from a monolayer without destroying their proteins?

I will do a cell culture, but I can not use trypsin-EDTA because it will lysate the membrane proteins and I'm interested in quantifying those proteins. Is there another way to take off the cells...

08 September 2017 1,459 6 View

I need help with the propagation of BCG (Mycobacterium bovis). Does anyone know which culture conditions need?

Propagation of BCG

08 September 2013 5,770 6 View

Differences between IL-12 p40 and IL-12 p70?

I wish to quantify cytokines in supernatant obtained lymphoproliferation assays but first I want to select which IL-12 should be tested.

08 September 2013 6,365 13 View

Fetal bovine serum can cause false positives in assays for human lymphocyte proliferation?

07 August 2013 9,530 13 View

Has anyone used Lipofectamine LTX + Plus Reagent for transfection in macrophages from THP-1 cells?

Tell me about your experiences in transfection please, thanks!

07 August 2013 9,835 3 View

How can Lymphocytes cells for transportation be preserved?

I need to transport cells from one country to another for further studies but is essential to preserve the viability.

07 August 2013 6,727 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View