I tried to follow the protocol of DSMZ and it didn't work. At the end, the cells were contaminated and I couldn't run my experiment until now. Does anyone have experience with this cell line?
The problem is: we follow the protocol of atcc but we fail to make her grow :) .... so, I wonder if anyone has experience with these cells ... and then give me a hint how I can subculture it profissionally :) ... Thanks for your interest Shiv