Hamed Abdelazim Helal

11 Questions 28 Answers 0 Followers

Questions related from Hamed Abdelazim Helal

Why does PC3 clones look smaller than its control when I use pcDNA vector?

I'm transfecting PC3 cell line with pcDNA vector carring different oligonucleotides and I did colony forming assay. The transfected cells make small colonies in comparision to control ones. If...

27 April 2015 1,255 3 View

Does metastasis represent an advantage or disadvantage during animal model study?

I am willing to do in vivo study (Xenograft) using 4T1 cell line (murine breast cancer cell line) and as I understand this cell line can highly metastasize to different organs. My goal of the...

12 September 2014 8,009 7 View

How long does it take to make a tumor xenograft using MDA-MB-468?

I am willing to do in vivo experiments using MDA-MB-468 and I wanna know how long it takes to form xenografts with a size of 2 cm. Thanks.

09 September 2014 301 6 View

How can I calculate the concentration of 50nM miRNA 100?

I am using MDA-MB-231 cell line in 6 well plate (300.000 cell/well) and I want to transfect them with 50nM and all the information I had is the mount is 5nmol. I am using RNAiMax.

01 August 2014 9,647 6 View

I want to run overnight western blot electrotransfer, what voltage should I use? The protein is p-ATM.

I always blot overnight 10 volt and then 1hr - 100 volt but the signal is always so weak ... any ideas?

13 July 2014 1,507 15 View

How can I carry out the MTT test with HCC1599?

I want to measure growth inhibition using HCC1599, but as it is a suspension cell line, I need to remove the medium before I add the MTT solvent without losing cells.

22 May 2014 1,267 5 View

Is it reasonable to compare Luminal A, Luminal B to triple negative breast cancer cell lines?

I am working in breast cancer research, comparing Luminal A, Luminal B to TNBC cell lines. I see that some of the Luminals share characteristics of TNBC. I wonder if we should compare all Luminals...

15 April 2014 3,977 10 View

What is the meaning of pCR in breast cancer patients?

Pathological complete response is a very important marker after chemotherapy. I want to understand what is the meaning of it.

01 December 2013 3,419 8 View

How can I subculture HCC1599 cell line?

I tried to follow the protocol of DSMZ and it didn't work. At the end, the cells were contaminated and I couldn't run my experiment until now. Does anyone have experience with this cell line?

15 October 2013 3,093 1 View

Are there different times to enter S phase in tumor cells?

I'm working with mamma cells, some of which grow slowly and others grow fast. I work mainly on the S phase, so I have to prepare my cells one day (24 hours) before the experiment and then run the...

21 August 2013 7,327 4 View

Are there differences between progression-free survival, relapse-free survival, and recurrence-free survival?

I'm reading on the medical reviews for TNBC and I'm a little bit confused with these terms.

12 May 2013 3,926 14 View