We have got an extensive list of IsoMir (cow genome used as map-file) after analyzing miRNA-seq data by miRAnalyzer (data were custom analyzed by s sequencing agency). Now we have the list in the following format:
SN microRNA Sample total UR total RC mature UR RC RC exact top Expr. exact /all mature* UR RC RC exact novel mature* exact/ total mature/ mature* align
1 bta-mir-191 MuBr 406 13499 bta-miR-191 400 13493 2202 FALSE 16.32 bta-mir-191-3p 4 4 1 TRUE 25 2202 details
2 bta-mir-1434 MuBr 605 12846 bta-miR-1434-5p 366 2900 1 FALSE 0.03 --- 0 0 0 FALSE 0 0 details
3 bta-mir-142 MuBr 490 8492 bta-miR-142-3p 348 6593 33 FALSE 0.5 bta-miR-142-5p 122 1872 21 FALSE 1.1 1.6 details
Please ignore the sample column. It is our code.
While clicking on the link "detail" on the last column, we are getting as below:
http://bioinfo5.ugr.es/miRanalyzer/miRanalyzer.php?id=71493031&align=hairpin&name=bta-mir-191
Please suggest howto proceed with this?
Take a look at the attached paper. I hope it can help
https://www.researchgate.net/publication/275049833_RNA-Seq_analysis_of_the_wild_barley_%28H._spontaneum%29_leaf_transcriptome_under_salt_stress
Article RNA-Seq analysis of the wild barley (H. spontaneum) leaf tra...
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