How might I enter new miRNA sequences to miRBase?

We have obtained some novel miRNA sequences of buffalo, following NGS of sRNA sequences (Ion Torrent platform). I have contacted the miRBase however, no communications received. Please let me know...

05 June 2015 8,950 9 View

How can I proceed with isoMir Data?

We have got an extensive list of IsoMir (cow genome used as map-file) after analyzing miRNA-seq data by miRAnalyzer (data were custom analyzed by s sequencing agency). Now we have the list in the...

05 June 2015 4,620 2 View

How can I proceed for Annotation and Characterization of novel miRNA?

We have sequenced miRNA of bubaline/buffalo leukocytes. Some novel miRNAs have been obtained. One example file has been attached. We are now blasting each of the novel miRNA sequence against the...

02 March 2015 5,891 5 View

How can I proceed for Annotation and Characterization of novel miRNA?

We have sequenced miRNA of bubaline/buffalo leukocytes. Some novel miRNAs have been obtained. One example file has been attached. We are now blasting each of the novel miRNA sequence against the...

02 March 2015 364 2 View

What is the mistake in this PERL code?

I am a novice in this field. Please help me identify the mistake in this code to use GetOptions. I am using Windows OS (Win8.1) #!/C:/Perl64/bin/perl require 5.16.3; # use strict; use...

05 June 2014 191 6 View

Which type of sequence should be used for phylogenetic tree construction, conserved or non conserved?

Suppose I have got a pair of sequences (100 amino acid each), one is conserved cds and another one is a non-conserved coding sequence. 1. Which one should be used for phylogeny? 2. Do these two...

05 June 2014 4,236 12 View

Why are common ancestors extinct?

Look at any phylogenetic tree we will find the internal nodes. However, do we have the common ancestors which are occupying those internal nodes in the real world? Sometimes we come up with...

03 April 2014 8,435 6 View

How is bioinformatics different from biostatistics?

It is well known that bioinformatics uses the knowledge of molecular biology, statistics, computer science and information technology (a part of computer science only). Can we say bioinformatics...

03 April 2014 6,954 7 View

Can someone recommend links to bioinformatics sites?

How useful is this site? http://bioinformaticssoftwareandtools.co.in/

07 August 2013 7,391 33 View

Real Time PCR

06 July 2013 2,316 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

No title

02 March 2021 3,060 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View