20 Questions 53 Answers 0 Followers
Questions related from C. S. Mukhopadhyay
We have got an extensive list of IsoMir (cow genome used as map-file) after analyzing miRNA-seq data by miRAnalyzer (data were custom analyzed by s sequencing agency). Now we have the list in the...
06 June 2015 4,689 2 View
We have obtained some novel miRNA sequences of buffalo, following NGS of sRNA sequences (Ion Torrent platform). I have contacted the miRBase however, no communications received. Please let me know...
06 June 2015 9,021 9 View
We have sequenced miRNA of bubaline/buffalo leukocytes. Some novel miRNAs have been obtained. One example file has been attached. We are now blasting each of the novel miRNA sequence against the...
03 March 2015 430 2 View
03 March 2015 5,951 5 View
I am a novice in this field. Please help me identify the mistake in this code to use GetOptions. I am using Windows OS (Win8.1) #!/C:/Perl64/bin/perl require 5.16.3; # use strict; use...
06 June 2014 259 6 View
Suppose I have got a pair of sequences (100 amino acid each), one is conserved cds and another one is a non-conserved coding sequence. 1. Which one should be used for phylogeny? 2. Do these two...
06 June 2014 4,301 12 View
It is well known that bioinformatics uses the knowledge of molecular biology, statistics, computer science and information technology (a part of computer science only). Can we say bioinformatics...
04 April 2014 7,014 7 View
Look at any phylogenetic tree we will find the internal nodes. However, do we have the common ancestors which are occupying those internal nodes in the real world? Sometimes we come up with...
04 April 2014 8,505 6 View
Why can lymphocytes be cultures in vitro, but not the other type of blood cells, like leukocytes (viz. granulocytes and monocytes) and erythrocytes? Although certain reports are there regarding...
08 August 2013 2,650 0 View
How useful is this site? http://bioinformaticssoftwareandtools.co.in/
08 August 2013 7,457 33 View
We are doing siRNA transfection in mammalian cells (suspension). The primers for target gene amplification were designed manually. As per the instruction provided by the siRNA generation kit, the...
07 July 2013 7,008 10 View
How it can be proved that Efficiency = 10(–1/Slope) – 1, in real time PCR assays. I need derivations.
07 July 2013 2,388 3 View
Subtractive genomics has been referred in bioinformatics papers. I am interested to know how to do such work?
02 February 2013 4,038 0 View
Please explain the meaning of Single Pass Read. Why do some database contain only single pass reads?
02 February 2013 9,904 0 View
I have got full length sequence of the coding sequence of a gene. BLASTn results are showing several predicted sequences vis-a-vis sequenced primary sequences of that cds belonging to different...
01 January 2013 9,731 24 View
The terms reliability of prediction and accuracy of predcition in genomic selection are frequently used. Plenty of papers are there discussing these points, however, I need the actual meaning of...
01 January 2013 7,306 8 View
Which site can be used as online tool for designing good degenerate primers?
01 January 2013 9,592 10 View
I was using the GENSCAN online program (http://genes.mit.edu/GENSCAN) for the prediction of gene (vertebrate sequence). I could not fully understand the meaning of these terms under the predicted...
11 November 2012 1,527 5 View
This query sounds very silly. But I was wondering if we can call NCBI or EMBL as server or not. Please explain.
10 October 2012 6,107 15 View
Someone suggested that the ratio between mean non-synonymous substitutions per non-synonymous site (dN) and mean synonymous substitutions per synonymous site (dS) should be calculated considering...
10 October 2012 7,256 9 View
