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Hi, I'm currently working on mineralization of SaOS-2 cell line in the presence of my drug of interest. Previously the drug which showed effect on the mineralization ability of the SaOS-2 cell...

11 December 2014 5,644 4 View

What is the importance of ligand partial atom charge and MD simulation?

Does the improper partial atom charge of the ligand have any effect on the MD simulation of the ligand protein complex? If so, will it will affect the calculation of potential mean force of the...

02 March 2014 4,566 5 View

Can anyone help with the differentiation of osteosarcoma?

I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.

01 February 2014 6,086 2 View

Can anyone help with Alizarin red staining protocol?

Is it normal for the 40mM alizarin red staining solution (pH 4.2), adjusted with ammonium hydroxide to be highly viscous and colloidal kind of nature. If not kindly do tell me how can I prepare it...

01 February 2014 5,704 1 View

Principle behind cell fixation?

Can anyone tell me, what is the principle behind cell fixation before any staining and also what happens to cell when we are treating it with the fixing agents like methanol, para-formaldehyde etc?

06 July 2013 4,917 9 View

Visualizing o-nitroaniline production in cell line

Is there any method available to stain the o-nitroaniline produced in the cell line?

06 July 2013 3,654 0 View

Endogenous control gene for qRT PCR?

Everyone knows that endogenous control is a yard stick for measuring the expression level of our gene of interest in control and treated samples. Do these endogenous controls vary during...

05 June 2013 7,671 3 View

Application of RT PCR for cell characterization?

I'm looking for a citation where established prime cell line is characterized using the real time PCR (from the gene expression profile of specific markers), rather than doing a flowcytometer. Is...

05 June 2013 6,977 0 View

Water mediated hydrogen bond

How can I measure the formation of water mediated hydrogen bonds formed between a ligand and a protein complex during simulation. I'm using gromacs 4.5v. Is there any specific way by which we can...

11 December 2012 3,405 1 View

Free energy calculation using gromacs

How can we calculate the ligand free energy binding for the trajectory obtained from gromacs ligand protein dynamics

10 November 2012 5,959 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View