Hi all, Can anyone help me in preventing the dystrophic/non-apatitc mineralization of SaOS-2 kept in the osteogenic media containing ascorbic acid, betaglycerophosphate and dexamethansone
Try titrating in Ahsg (fetuin) into the culture media.
Hi, I'm currently working on mineralization of SaOS-2 cell line in the presence of my drug of interest. Previously the drug which showed effect on the mineralization ability of the SaOS-2 cell...
11 December 2014 5,731 4 View
Does the improper partial atom charge of the ligand have any effect on the MD simulation of the ligand protein complex? If so, will it will affect the calculation of potential mean force of the...
02 March 2014 4,686 5 View
I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.
01 February 2014 6,176 2 View
Is it normal for the 40mM alizarin red staining solution (pH 4.2), adjusted with ammonium hydroxide to be highly viscous and colloidal kind of nature. If not kindly do tell me how can I prepare it...
01 February 2014 5,785 1 View
Can anyone tell me, what is the principle behind cell fixation before any staining and also what happens to cell when we are treating it with the fixing agents like methanol, para-formaldehyde etc?
06 July 2013 5,026 9 View
Is there any method available to stain the o-nitroaniline produced in the cell line?
06 July 2013 3,746 0 View
Everyone knows that endogenous control is a yard stick for measuring the expression level of our gene of interest in control and treated samples. Do these endogenous controls vary during...
05 June 2013 7,755 3 View
I'm looking for a citation where established prime cell line is characterized using the real time PCR (from the gene expression profile of specific markers), rather than doing a flowcytometer. Is...
05 June 2013 7,064 0 View
How can I measure the formation of water mediated hydrogen bonds formed between a ligand and a protein complex during simulation. I'm using gromacs 4.5v. Is there any specific way by which we can...
11 December 2012 3,497 1 View
How can we calculate the ligand free energy binding for the trajectory obtained from gromacs ligand protein dynamics
10 November 2012 6,069 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View