I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.
3) The tankyrase-specific inhibitor JW74 affects cell cycle progression and induces apoptosis and differentiation in osteosarcoma cell lines.
Wessel Stratford E, Daffinrud J, Munthe E, Castro R, Waaler J, Krauss S, Myklebost O.
Cancer Med. 2013 Dec 17. doi: 10.1002/cam4.170
4) Osteosarcoma cells promote the production of pro-tumor cytokines in mesenchymal stem cells by inhibiting their osteogenic differentiation through the TGF-β/Smad2/3 pathway.
hi raj. in morphological ground, the nuclei of the neoplastic cell is a good reference of the differentiation; hipercromatic and pleomorphic nuclei and neoplastic osteoid is a signal of osteoblastic differentiation, if the tumor cell have a big nuclei, nucleoli, clear citoplasm or binucleation in a chondroid stroma, the differentiation is chondroid and finally a fusiform nuclei in a fibrous stroma is signal of fibroblastic differentiation.