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Hi, I'm currently working on mineralization of SaOS-2 cell line in the presence of my drug of interest. Previously the drug which showed effect on the mineralization ability of the SaOS-2 cell...

11 December 2014 5,644 4 View

How can I prevent the occurrence of dystrophic/non-apatitic mineralization of SaOS-2 ?

Hi all, Can anyone help me in preventing the dystrophic/non-apatitc mineralization of SaOS-2 kept in the osteogenic media containing ascorbic acid, betaglycerophosphate and dexamethansone

11 December 2014 5,402 1 View

What is the importance of ligand partial atom charge and MD simulation?

Does the improper partial atom charge of the ligand have any effect on the MD simulation of the ligand protein complex? If so, will it will affect the calculation of potential mean force of the...

02 March 2014 4,566 5 View

Can anyone help with the differentiation of osteosarcoma?

I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.

01 February 2014 6,086 2 View

Can anyone help with Alizarin red staining protocol?

Is it normal for the 40mM alizarin red staining solution (pH 4.2), adjusted with ammonium hydroxide to be highly viscous and colloidal kind of nature. If not kindly do tell me how can I prepare it...

01 February 2014 5,704 1 View

Principle behind cell fixation?

Can anyone tell me, what is the principle behind cell fixation before any staining and also what happens to cell when we are treating it with the fixing agents like methanol, para-formaldehyde etc?

06 July 2013 4,917 9 View

Visualizing o-nitroaniline production in cell line

Is there any method available to stain the o-nitroaniline produced in the cell line?

06 July 2013 3,654 0 View

Endogenous control gene for qRT PCR?

Everyone knows that endogenous control is a yard stick for measuring the expression level of our gene of interest in control and treated samples. Do these endogenous controls vary during...

05 June 2013 7,671 3 View

Water mediated hydrogen bond

How can I measure the formation of water mediated hydrogen bonds formed between a ligand and a protein complex during simulation. I'm using gromacs 4.5v. Is there any specific way by which we can...

11 December 2012 3,405 1 View

Free energy calculation using gromacs

How can we calculate the ligand free energy binding for the trajectory obtained from gromacs ligand protein dynamics

10 November 2012 5,959 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View