Hi, I'm currently working on mineralization of SaOS-2 cell line in the presence of my drug of interest. Previously the drug which showed effect on the mineralization ability of the SaOS-2 cell line is now showing no effect at all.
Do the cell confluence alters SaOS-2 cell line drug sensitivity? Any one has earlier experience on this ? please share.
In general, the degree of cellularity affects the susceptibility to the exogenous compound probably due to the difference of the permeability to the cells cultured in 2-D culture. From my experience, the pharmacological effect should be evaluated under the cellularity of 70-80%.
I agree with earlier comments. Increase in cell density in culture is known to decrease sensitivity to a number of agents and increase cell survival. Therefore, it may be desirable to fix the concentration of compound of interest and vary cell density from 25% to 100% to identify the optimum cell density for proposed study.
Hi! which drugs and concentrations are you using to induce mineralization on SAOS-2 cells?
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