Is it normal for the 40mM alizarin red staining solution (pH 4.2), adjusted with ammonium hydroxide to be highly viscous and colloidal kind of nature. If not kindly do tell me how can I prepare it in solution form.
Antonio M Spadaccino
Hi, I found on an old chemistry book that the correct pH for alizarin red is between 6.3 and 6.5 stirred thoroughly and adjusted with HCl or 95% alcohol all the sections fixatives have to be neutral to avoid pH swings and half the staining time if the sections contain lots of calcium rinsing in acid alcohol (1% acetic acid) increases contrast and fixatives containing acid should be avoided. short rinses to avoid loosing the stained calcium. Hope this works
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