13 Questions 10 Answers 0 Followers
Questions related from Raj Kumar Chinnadurai
Hi, I'm currently working on mineralization of SaOS-2 cell line in the presence of my drug of interest. Previously the drug which showed effect on the mineralization ability of the SaOS-2 cell...
12 December 2014 5,707 4 View
Hi all, Can anyone help me in preventing the dystrophic/non-apatitc mineralization of SaOS-2 kept in the osteogenic media containing ascorbic acid, betaglycerophosphate and dexamethansone
12 December 2014 5,467 1 View
Does the improper partial atom charge of the ligand have any effect on the MD simulation of the ligand protein complex? If so, will it will affect the calculation of potential mean force of the...
03 March 2014 4,655 5 View
Is it normal for the 40mM alizarin red staining solution (pH 4.2), adjusted with ammonium hydroxide to be highly viscous and colloidal kind of nature. If not kindly do tell me how can I prepare it...
02 February 2014 5,761 1 View
I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.
02 February 2014 6,152 2 View
Can anyone tell me, what is the principle behind cell fixation before any staining and also what happens to cell when we are treating it with the fixing agents like methanol, para-formaldehyde etc?
07 July 2013 4,994 9 View
Is there any method available to stain the o-nitroaniline produced in the cell line?
07 July 2013 3,721 0 View
Everyone knows that endogenous control is a yard stick for measuring the expression level of our gene of interest in control and treated samples. Do these endogenous controls vary during...
06 June 2013 7,731 3 View
I'm looking for a citation where established prime cell line is characterized using the real time PCR (from the gene expression profile of specific markers), rather than doing a flowcytometer. Is...
06 June 2013 7,036 0 View
How can I measure the formation of water mediated hydrogen bonds formed between a ligand and a protein complex during simulation. I'm using gromacs 4.5v. Is there any specific way by which we can...
12 December 2012 3,470 1 View
I am doing MTT assay for the drug in IMR-32 cell line. Initially I incubated the cells in 96 well plates for 24 hrs followed by drug treatment for 24 hrs after washing the wells with sterile PBS....
11 November 2012 1,812 10 View
How can we calculate the ligand free energy binding for the trajectory obtained from gromacs ligand protein dynamics
11 November 2012 6,035 3 View
How can i measure the hydrophobic interaction between the ligand and protein using gromacs
11 November 2012 3,444 3 View
