I am going through the experiment in which MC3T3E1 cells are induced for osteogenesis. I use inducing factors such as ascorbic acid(50uM), Beta-Glycerophosphate(10mM) and dexamethasone(100nM). After a few days I find cells detached. If I don't use dexamthasone in the combination, then no cell gets detached. But I can't get osteogenesis. I am using alpha MEM with 10%FBS, 1X antibiotics and 1X glutamine. Can you please give me some idea to overcome that.

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