Can anyone give me the reference number or percentage of mesenchymal stem cells in total bone marrow cells excluding RBCs?
The trabecular bone volume decreases on advancing the ages. It is clear that aged mice have lower bone volume than younger mice. By reading many articles I am still having confusion between...
10 November 2017 5,267 2 View
Hello everyone, I am planning for a work related to mesenchymal to epithelial transition(MET). Actually I want to induce MET using the mesenchymal stem cells by some gene transfections. I am not...
10 November 2015 3,118 5 View
I am going through the experiment in which MC3T3E1 cells are induced for osteogenesis. I use inducing factors such as ascorbic acid(50uM), Beta-Glycerophosphate(10mM) and dexamethasone(100nM)....
11 December 2014 8,109 7 View
I would like to know using Alpha MEM having ascorbic acid is good idea or not for mesenchymal stem cells culture. I generally use mice/rat bone marrow stromal cells for MSCs. using alpha MEM...
10 November 2014 5,779 35 View
Can anyone provide me a protocol for isolating mRNA and protein from the bone marrow of mice, rat and humans? I don't want to use any isolating kit.
08 September 2014 2,457 2 View
I am having problem of mouse bone marrow stromal cells primary culture. First 2-3 days I see cells very healthy but then slowly hazzy and dying. I use alpha MEM media with 10% FBS and 1x glutamine...
07 August 2014 5,135 7 View
Hello everyone, There are lots of literature those explain about the senescence of mesenchymal stem cells by hyperglycemic conditions in in-vitro conditions. Also we can find the papers explaining...
01 January 1970 5,685 6 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View