Sher Bahadur Poudel

8 Questions 24 Answers 0 Followers

Questions related from Sher Bahadur Poudel

How the trabecular bone volume differs between young and adult mice?

The trabecular bone volume decreases on advancing the ages. It is clear that aged mice have lower bone volume than younger mice. By reading many articles I am still having confusion between...

11 November 2017 5,245 2 View

What is the percentage of Mesenchymal stem cells in total Bone marrow cells?

Can anyone give me the reference number or percentage of mesenchymal stem cells in total bone marrow cells excluding RBCs?

09 September 2016 865 0 View

Which cell lines are good for inducing mesenchymal to epithelial transition work?

Hello everyone, I am planning for a work related to mesenchymal to epithelial transition(MET). Actually I want to induce MET using the mesenchymal stem cells by some gene transfections. I am not...

11 November 2015 3,097 5 View

How can I overcome MC3T3E1 detachment after Dexamethasone treatment?

I am going through the experiment in which MC3T3E1 cells are induced for osteogenesis. I use inducing factors such as ascorbic acid(50uM), Beta-Glycerophosphate(10mM) and dexamethasone(100nM)....

12 December 2014 8,085 7 View

What is appropriate media for MSCs culture?

I would like to know using Alpha MEM having ascorbic acid is good idea or not for mesenchymal stem cells culture. I generally use mice/rat bone marrow stromal cells for MSCs. using alpha MEM...

11 November 2014 5,754 35 View

What is the best method for isolating protein and mRNA from bone marrow?

Can anyone provide me a protocol for isolating mRNA and protein from the bone marrow of mice, rat and humans? I don't want to use any isolating kit.

09 September 2014 2,431 2 View

What is the best method to analyze mouse bone marrow stromal cell culture?

I am having problem of mouse bone marrow stromal cells primary culture. First 2-3 days I see cells very healthy but then slowly hazzy and dying. I use alpha MEM media with 10% FBS and 1x glutamine...

08 August 2014 5,108 7 View

Can anyone suggest the literature explaining the senescence of bone marrow mesenchymal stem cells of diabetic mice model?

Hello everyone, There are lots of literature those explain about the senescence of mesenchymal stem cells by hyperglycemic conditions in in-vitro conditions. Also we can find the papers explaining...

01 January 1970 5,663 6 View