How can I find the density of bands?

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Can any one try to solve the problem fir tricine SDS electrophoreses?

Dear all With respect to above mentioned subject, we use tricine SDS electrophoresis to resolve small peptides. Earlier separation was very good and getting sharp bands. Now we are facing problem...

07 August 2017 8,105 9 View

How can I stabilize the method for gene copy index estimation?

Hello friends. I am optimizing the method for estimating gene copy index by using Beta actin as house keeping gene. In each trail using same genomic DNA, I am getting different CT value. Can any...

11 December 2015 8,277 6 View

How can I get 100% efficiency in qPCR for house keeping gene Beta actin?

Hello friends I am trying to estimate gene copy index by using B-actin as a house keeping gene. I am getting 100% efficiency for my desired gene but failing to get for B-actin. Can any one give me...

11 December 2015 1,275 5 View

How to study liver histology?

I prepared liver section and also took photographs. Now I don't know how to analyse the histology. What are the parameters we need to study including necrosis and hemorrhage? How to find out...

07 August 2013 4,489 7 View

How to recover sephadex?

I am using sephadex LH 20 to separate the natural product from ethanol extract of a plant. For a single use only, sephadex turned red in color. For further use, I need to recover the matrix. So,...

07 August 2013 2,047 1 View

How can I find out the presence of single or multiple compound in elute?

I did column chromatography to separate the compounds present in plant extract using sephadex LH 20 as stationary phase and 100 % as the solvent system. Now I need to find out whether the elute...

07 August 2013 6,601 3 View

Does anybody know how to use SPSS?

Kindly give me a flow chart or suggest me how to use SPSS software for ANOVA and many statistical analysis.

04 May 2013 7,258 9 View

Need of preparative HPLC.

I am working on natural products and I am facing too many problems while separating compounds from crude plant extract. I tried all the ways, but it was no use. Now the ultimate step is to...

02 March 2013 7,264 18 View

What are the possible secondary metabolites to petroleum ether while doing extraction?

Before going to extraction with polar solvents, we need to defat the plant materials with lw polar solvents like petroleum ether. My question is, what are the possible secondary metabolites to...

02 March 2013 7,670 7 View

How to isolate phenolic compounds from ethanolic extract.

I am facing too many problems while isolating pure compounds. We don't have a facility for HPLC. How can I isolate pure compounds (Phenolics including flavonoids)?

01 February 2013 279 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View