How can I find the density of bands?

More Rajesh K P's questions See All

Can any one try to solve the problem fir tricine SDS electrophoreses?

Dear all With respect to above mentioned subject, we use tricine SDS electrophoresis to resolve small peptides. Earlier separation was very good and getting sharp bands. Now we are facing problem...

07 August 2017 8,233 9 View

How can I stabilize the method for gene copy index estimation?

Hello friends. I am optimizing the method for estimating gene copy index by using Beta actin as house keeping gene. In each trail using same genomic DNA, I am getting different CT value. Can any...

11 December 2015 8,398 6 View

How can I get 100% efficiency in qPCR for house keeping gene Beta actin?

Hello friends I am trying to estimate gene copy index by using B-actin as a house keeping gene. I am getting 100% efficiency for my desired gene but failing to get for B-actin. Can any one give me...

11 December 2015 1,428 5 View

How to study liver histology?

I prepared liver section and also took photographs. Now I don't know how to analyse the histology. What are the parameters we need to study including necrosis and hemorrhage? How to find out...

07 August 2013 4,613 7 View

How to recover sephadex?

I am using sephadex LH 20 to separate the natural product from ethanol extract of a plant. For a single use only, sephadex turned red in color. For further use, I need to recover the matrix. So,...

07 August 2013 2,173 1 View

How can I find out the presence of single or multiple compound in elute?

I did column chromatography to separate the compounds present in plant extract using sephadex LH 20 as stationary phase and 100 % as the solvent system. Now I need to find out whether the elute...

07 August 2013 6,708 3 View

Does anybody know how to use SPSS?

Kindly give me a flow chart or suggest me how to use SPSS software for ANOVA and many statistical analysis.

04 May 2013 7,376 9 View

Need of preparative HPLC.

I am working on natural products and I am facing too many problems while separating compounds from crude plant extract. I tried all the ways, but it was no use. Now the ultimate step is to...

02 March 2013 7,376 18 View

What are the possible secondary metabolites to petroleum ether while doing extraction?

Before going to extraction with polar solvents, we need to defat the plant materials with lw polar solvents like petroleum ether. My question is, what are the possible secondary metabolites to...

02 March 2013 7,794 7 View

How to isolate phenolic compounds from ethanolic extract.

I am facing too many problems while isolating pure compounds. We don't have a facility for HPLC. How can I isolate pure compounds (Phenolics including flavonoids)?

01 February 2013 408 5 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Anybody can assist me in Protein and magnetic beads seperation?

Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...

02 July 2024 5,502 4 View

How accurate is Image J for SDS bands analysis?

I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...

01 July 2024 1,841 3 View

How to troubleshoot error E1 on Biorad Powerpac Basic despite using sufficient and fresh buffer?

I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...

01 July 2024 7,104 1 View

Thin DNA gel loading sample tips?

I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...

01 July 2024 2,124 2 View