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Questions related from Rajesh K P
Dear all With respect to above mentioned subject, we use tricine SDS electrophoresis to resolve small peptides. Earlier separation was very good and getting sharp bands. Now we are facing problem...
08 August 2017 8,202 9 View
Hello friends I am trying to estimate gene copy index by using B-actin as a house keeping gene. I am getting 100% efficiency for my desired gene but failing to get for B-actin. Can any one give me...
12 December 2015 1,400 5 View
Hello friends. I am optimizing the method for estimating gene copy index by using Beta actin as house keeping gene. In each trail using same genomic DNA, I am getting different CT value. Can any...
12 December 2015 8,369 6 View
Hi, recently I saw one paper stating that, they estimated the density of the bands on SDS-PAGE using Adobe photoshop application. Anybody knows how to do that? Paper has been attached.
06 June 2014 8,250 2 View
I prepared liver section and also took photographs. Now I don't know how to analyse the histology. What are the parameters we need to study including necrosis and hemorrhage? How to find out...
08 August 2013 4,575 7 View
I did column chromatography to separate the compounds present in plant extract using sephadex LH 20 as stationary phase and 100 % as the solvent system. Now I need to find out whether the elute...
08 August 2013 6,679 3 View
I am using sephadex LH 20 to separate the natural product from ethanol extract of a plant. For a single use only, sephadex turned red in color. For further use, I need to recover the matrix. So,...
08 August 2013 2,142 1 View
Kindly give me a flow chart or suggest me how to use SPSS software for ANOVA and many statistical analysis.
05 May 2013 7,348 9 View
I am working on natural products and I am facing too many problems while separating compounds from crude plant extract. I tried all the ways, but it was no use. Now the ultimate step is to...
03 March 2013 7,346 18 View
Before going to extraction with polar solvents, we need to defat the plant materials with lw polar solvents like petroleum ether. My question is, what are the possible secondary metabolites to...
03 March 2013 7,759 7 View
I am facing too many problems while isolating pure compounds. We don't have a facility for HPLC. How can I isolate pure compounds (Phenolics including flavonoids)?
02 February 2013 378 5 View
