How to recover sephadex?

08 August 2013 1 2K Report

I am using sephadex LH 20 to separate the natural product from ethanol extract of a plant. For a single use only, sephadex turned red in color. For further use, I need to recover the matrix. So, can anyone tell me how to remove the color from seohadex LH20?

Robin Joshi

Regenerate your sephadex. Wash your column with mild acid. then wash with water followed by your desired mobile phase.

Gudluck

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