Can any one try to solve the problem fir tricine SDS electrophoreses?

Dear all With respect to above mentioned subject, we use tricine SDS electrophoresis to resolve small peptides. Earlier separation was very good and getting sharp bands. Now we are facing problem...

07 August 2017 8,105 9 View

How can I stabilize the method for gene copy index estimation?

Hello friends. I am optimizing the method for estimating gene copy index by using Beta actin as house keeping gene. In each trail using same genomic DNA, I am getting different CT value. Can any...

11 December 2015 8,277 6 View

How can I get 100% efficiency in qPCR for house keeping gene Beta actin?

Hello friends I am trying to estimate gene copy index by using B-actin as a house keeping gene. I am getting 100% efficiency for my desired gene but failing to get for B-actin. Can any one give me...

11 December 2015 1,275 5 View

How can I find the density of bands?

Hi, recently I saw one paper stating that, they estimated the density of the bands on SDS-PAGE using Adobe photoshop application. Anybody knows how to do that? Paper has been attached.

05 June 2014 8,154 2 View

How to study liver histology?

I prepared liver section and also took photographs. Now I don't know how to analyse the histology. What are the parameters we need to study including necrosis and hemorrhage? How to find out...

07 August 2013 4,489 7 View

How to recover sephadex?

I am using sephadex LH 20 to separate the natural product from ethanol extract of a plant. For a single use only, sephadex turned red in color. For further use, I need to recover the matrix. So,...

07 August 2013 2,047 1 View

How can I find out the presence of single or multiple compound in elute?

I did column chromatography to separate the compounds present in plant extract using sephadex LH 20 as stationary phase and 100 % as the solvent system. Now I need to find out whether the elute...

07 August 2013 6,601 3 View

Need of preparative HPLC.

I am working on natural products and I am facing too many problems while separating compounds from crude plant extract. I tried all the ways, but it was no use. Now the ultimate step is to...

02 March 2013 7,264 18 View

What are the possible secondary metabolites to petroleum ether while doing extraction?

Before going to extraction with polar solvents, we need to defat the plant materials with lw polar solvents like petroleum ether. My question is, what are the possible secondary metabolites to...

02 March 2013 7,670 7 View

How to isolate phenolic compounds from ethanolic extract.

I am facing too many problems while isolating pure compounds. We don't have a facility for HPLC. How can I isolate pure compounds (Phenolics including flavonoids)?

01 February 2013 279 5 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View