Phosphorylation sites prediction?

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Why do these abnormal charge/discharge curves of coin cell batteries appear?

As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...

24 July 2024 6,930 1 View

How can I prevent protein subunits within a protein complex from dissociating without altering the overall structure?

I have a protein complex in which some dimers easily dissociate. What methods can I use to stabilize these dimers within the complex without altering the overall structure of the complex? Will...

16 July 2024 2,757 2 View

How to predict Protein structure and choose N or C terminal tagging?

Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...

24 June 2024 8,622 6 View

What can we get from ZFC/FC curves and what is the reason behind?

We have measured the ZFC/FC curve under 100Oe, there is splitting happens and there is another turning point happens to around 25K, why it is increased again with decreasing temperature?

11 June 2024 9,603 1 View

How to feed DICOM images into deep learning model?

Hello. Hope you are doing fine. I am an undergrad student . I am working on a project. Can you guide me in a query. I have CT scan dataset in the form of DICOM meaning for a single CT scan I have...

10 June 2024 7,306 1 View

How can I find someone to present on my behalf?

I need assistance. I submitted a paper to the DEXA2024 conference, and it has been accepted. However, I am unable to attend the conference held in Italy. Now, I am looking for a partner whose...

12 May 2024 8,431 0 View

How to convert the mat to a raster?

I have a mat that have 3 data about grid_X、grid_Y and Mb,i want save the tif in ArcGIS use the mat. mat file in the attachment.

09 May 2024 1,541 1 View

About patient psychology, what are the current research focuses and future directions?

I'm eager to hear your professional views about patient psychology!

04 May 2024 4,497 2 View

Can intron 1 site be used for CRSIPR Cas9 mediated Knock in?

hey, I am designing gRNA target site to check the endogenous expression of protein in zebrafish. For this I am using chop-chop site. But most of the sites are in exonic region. I couldn't find...

13 April 2024 3,757 3 View

Oxidation of SWCNT-COOH?

Hello everyone. Could someone advise on the likelihood of high-purity SWCNT-COOH undergoing oxidation when stored in a refrigerator? I suspect that my SWCNT-COOH may have been oxidized due to the...

31 March 2024 2,524 0 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

PVDF or nitrocellulose to visualize phosphorylated proteins?

Which membrane material is the best for capturing and visualizing phosphorylated proteins in a western blot? Or is there no difference?

05 June 2024 9,502 2 View

What are the options for testing the phosphorylation of HP1a?

I have used CSII to phosphorylate HP1a, in vitro. Now, to test this phosphorylation, I have options such as using an anti-serine phospho antibody or mass spectrometry. Has anyone ever tested an...

08 April 2024 1,952 2 View

Recommendations for phospho-serine and/or phospho-threonine antibody?

Can anyone recommend a "good" general phospho-serine and/or phospho-threonine antibody for use after immunoprecipitation in a western blot? There are a lot of expensive offers online with...

22 February 2024 1,080 0 View

How well can be p-c-Jun detected in Westernblot?

Regarding the expression of phosphorylated c-Jun (p-c-Jun) in kidney tissue observed through Western blotting, even under high sensitivity settings and after 30 minutes, the detection quantity was...

20 February 2024 9,150 2 View

Which phosphor antibodies to choose to study the hypo and hyperphosphorylation state of the SRSF1 protein?

I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...

11 February 2024 7,242 3 View

How to treat cells with Kinase Inhibitor to analyze if extra IP bands are due to phosphorylation?

Hey everyone, I bought this kinase inhibitor (Sigma 20-116). It did not come with any instructions/protocols in terms of treating cells with it. I plan to use the cell lysate for an IP. When...

08 February 2024 7,493 0 View

Collapsing overlapping peptide fragments in LC-MS/MS data?

Hello, I am having trouble finding a method to collapse log fold change data of peptides determined from mass spec that have the same peptide sequence but differ in the number of PTM events. An...

04 February 2024 166 0 View