How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Can anyone help me with the problem of hyperelastic coefficients in abaqus?

Hello Everyone, I have done a uniaxial test on rubber and data (attached with the post) has been used in Abaqus to get YEOH's coefficient. But I am getting a warning that it is unstable? Can...

04 February 2021 5,975 3 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

Submission of the Materials Studio jobs to the remote Linux cluster?

I am doing some simulations using the Forcite module in Materials Studio. As the speed of this calculation on my workstation computer is unacceptably slow, I am trying to submit my job to the...

14 January 2021 8,653 1 View

Can you please help with the problem modeling a damper in ABAQUS?

Hello everyone, I am trying to model a damper in ABAQUS. I have obtained hyperelastic properties using a uniaxial tensile test and viscoelastic properties by relaxation test conducted in the...

09 January 2021 4,515 1 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

Is there any relationship/correaltion between 1) elastic modulus and adhesion or 2) elastic modulus and density of solid material or polymers?

21 December 2020 1,853 3 View

Can anyone help me with the constraint between two rods?

Hello Everyone, I am trying to model a wire mesh in abaqus. I have modeled it with 1mm circular cylinder. I want to give a tie constraint to the perpendicular wires. I am unable to find it in...

16 December 2020 3,572 6 View

How to convert Time Domain DMA data to prony series?

Hello Everyone, I have Time Domain DMA data of rubber i.e. loss modulus and storage modulus varying with time with constant 1 hz frequency. I need to convert it into prony coefficient. I need to...

09 December 2020 5,211 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View

Is it possible that the phosphorylated proteins mobilize faster than the non-phosphorylated ones?

The band downshifts on Western-blot, after kinase assay treatment.

23 December 2020 7,959 3 View

Terminal 5' phosphate in DNA simulation using AMBER FF in Gromacs?

How to add a new termini database entries in amber FF for phosphorylated at 5'- and 3'-termini of DNA? Pdb2gmx is not able to covert pdb to gro files could you please help me?

22 September 2020 8,356 7 View

Where I could find a phospho-CPT1A antibody ?

I started working on CPT1A enzyme and particularly interested in the post-translational modifications of CPT1A. So I was looking for phospho-CPT1A antibody and couldn't find any. Can anybody tell...

09 September 2020 1,127 3 View

Best way to confirm phospho-mutants?

We have generated a few phosphomimetics mutants of our protein (constitutively active threonine and serine site). We are wondering about what is the best way to confirm the mutants have active...

25 August 2020 4,000 2 View

Multi band on western blot for glucocorticoid receptor. Which band(s) should I select for quantitative study?

Hello I can see different bands for phosphorylated glucocorticoid receptor protein on the blot membrane. I get confused which band(s) should I select? Can I select the whole areas where the bands...

17 August 2020 6,771 8 View

What do you suggest to investigate cell signaling with the small number of cells?

I have a plan to investigate cell signaling on NKG2D-expressing CD4. For this matter, I plan to isolate primary cells from patients and use western blot technique to measure the expression level...

08 August 2020 911 5 View

The meaning of protein phosphorylation to bioscience?

Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did...

23 July 2020 7,135 3 View